Sean H Johnson

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Unmarked gene modifications are desirable for various genetic analyses; however, they have been difficult to construct without selectable markers. We describe here a new genetic method for constructing unmarked mutants in Streptococcus mutans. The desired mutant allele is first constructed and introduced into the strain by cotransformation with pGhost4, a(More)
For the last several years, I have been studying astronomical nebulae, and the work has slowly led to a desire to know more about atomic structure calculations. The state of these nebulae (temperature, densities, abundances, etc) are typically constrained through the use of diagnostic emission line ratios as detailed in Osterbrock & Ferland (2006). For(More)
  • Rachelle Boyson, Nich Eggert, +7 authors Tony Zavala
  • 2014
The work to be presented here is the result of the collaborative efforts of ten students of syntax. The aim here—as it is of all syntactic analyses—is to be able to account for a construction while working within the domain of a certain syntactic theory, upholding the tenets of said theory, making minimal adjustments to it only as necessary, and creating(More)
Eosinophils contribute to immune regulation and wound healing/fibrosis in various diseases, including asthma. Growing appreciation for the role of activin A in such processes led us to hypothesize that eosinophils are a source of this transforming growth factor-ß superfamily member. Tumor necrosis factor-α (TNF) induces activin A by other cell types and is(More)
Nicotinamide phosphoribosyltransferase (NAMPT), the key NAD(+) biosynthetic enzyme, has two different forms, intra- and extracellular (iNAMPT and eNAMPT), in mammals. However, the significance of eNAMPT secretion remains unclear. Here we demonstrate that deacetylation of iNAMPT by the mammalian NAD(+)-dependent deacetylase SIRT1 predisposes the protein to(More)
OBJECTIVE Recently, increased attention has been focused on the diagnosis of the most prevalent category of fetal alcohol spectrum disorders, alcohol-related neurodevelopmental disorders (ARNDs). In 2013, proposed criteria for neurodevelopmental disorders associated with prenatal alcohol exposure (ND-PAE) were included in the appendix of the latest revision(More)
Current philosophy and practice urge, even require for funding, that programs be empirically based and grounded in empirically proven emerging, promising, or best practices. In most of the human services, including youth programs, services, and practices, this requirement is a goal as well as an ideal. Empirical research and evaluation can be used in many(More)
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