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Both the brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) RNA-dependent RNA polymerases (RdRps) were found to recognize the BMV core subgenomic promoter in the same manner, requiring specific functional groups at positions -17, -14, -13, and -11 relative to the subgenomic initiation site (+1). For CCMV subgenomic RNA synthesis, both RdRps(More)
Short regions of (-)-strand brome mosaic virus (BMV) RNA3, proscripts, were shown to direct accurate in vitro synthesis of (+)-strand subgenomic RNA by the BMV RNA-dependent RNA polymerase (RdRp), facilitating characterization of the sequences and/or structures directing subgenomic RNA synthesis. Proscripts retaining fewer than 8-nt of an 18-nt(More)
RNA templates of 33 nucleotides containing the brome mosaic virus (BMV) core subgenomic promoter were used to determine the promoter elements recognized by the BMV RNA-dependent RNA polymerase (RdRp) to initiate RNA synthesis. Nucleotides at positions -17, -14, -13, and -11 relative to the subgenomic initiation site must be maintained for interaction with(More)
The brome mosaic virus (BMV) RNA-dependent RNA polymerase (RdRp) directs template-specific synthesis of (-)-strand genomic and (+)-strand subgenomic RNAs in vitro. Although the requirements for (-)-strand RNA synthesis have been characterized previously, the mechanism of subgenomic RNA synthesis has not. Mutational analysis of the subgenomic promoter(More)
We are designing the Keck Cosmic Web Imager (KCWI) as a new facility instrument for the Keck II telescope at the W. M. Keck Observatory (WMKO). KCWI is based on the Cosmic Web Imager (CWI), an instrument that has recently had first light at the Hale Telescope. KCWI is a wide-field integral-field spectrograph (IFS) optimized for precision sky limited(More)
RNA replication, a process of fundamental importance for pathogenesis by many viruses, remains poorly understood at the mechanistic level because relatively few of the responsible enzymes have been purified and characterized biochemically. Partially purified RNA-dependent RNA polymerase (RdRp) from brome mosaic virus (BMV)-infected barley leaves is able to(More)
This paper introduces a novel application of IBR technology for efficient rendering of high quality CG and live action stereoscopic sequences. Traditionally, IBR has been applied to render novel views using image and depth based representations of the plenoptic functions. In this work, we present a restricted form of IBR in which lower resolution images for(More)
This paper describes the as-built performance of MOSFIRE, the multi-object spectrometer and imager for the Cassegrain focus of the 10-m Keck 1 telescope. MOSFIRE provides near-infrared (0.97 to 2.41 m) multi-object spectroscopy over a 6.1' x 6.1' field of view with a resolving power of R~3,500 for a 0.7" (0.508 mm) slit (2.9 pixels in the dispersion(More)
MOSFIRE is a unique multi-object spectrometer and imager for the Cassegrain focus of the 10 m Keck 1 telescope. A refractive optical design provides near-IR (0.97 to 2.45 μm) multi-object spectroscopy over a 6.14' x 6.14' field of view with a resolving power of R~3,270 for a 0.7" slit width (2.9 pixels in the dispersion direction), or imaging over a field(More)
MOSFIRE, the multi-object spectrometer for infra-red exploration, is a near-IR (0.97-2.45 micron) spectrograph and imager for the Cassegrain focus of the Keck I telescope. The optical design provides imaging and multi-object spectroscopy over a field of view (FOV) of 6.14' x 6.14' with a resolving power of R~3,270 for a slit width of 0.7 arc seconds (2.9(More)