The objectives of this study were to estimate the prevalence of Mycobacterium avium subsp. paratuberculosis (MAP) among deer and rabbits surrounding infected and noninfected Minnesota dairy farms using fecal culture, and to describe the frequency that farm management practices were used that could potentially lead to transmission of infection between these… (More)
BACKGROUND Mycobacterium avium subsp. paratuberculosis (MAP) persistently infects intestines and mesenteric lymph nodes leading to a prolonged subclinical disease. The MAP genome sequence was published in 2005, yet its transcriptional organization in natural infection is unknown. While prior research analyzed regulated gene sets utilizing defined, in vitro… (More)
OBJECTIVE To characterize the risk of interactions that may lead to the transmission of Mycobacterium bovis between cattle and white-tailed deer (Odocoileus virginianus) on farms in northern Minnesota. SAMPLE 53 cattle farms in northwestern Minnesota adjacent to an area where bovine tuberculosis-infected cattle and deer were detected. PROCEDURES A… (More)
We describe eight novel microsatellite loci for the tui, Prosthemadera novaeseelandiae, and the cross-amplification of six polymorphic loci developed for the bellbird (Anthornis melanura). Fifty tui samples from Tawharanui Regional Park, New Zealand, were genotyped using the 14 loci described. Numbers of alleles ranged from 3 to 16. Observed and expected… (More)
The objective was to evaluate the survival of Mycobacterium paratuberculosis (Map) in naturally infected dairy cows feces under long periods of freezing at -18°C and -70°C. Samples were collected from cows previously tested positive with serum ELISA or fecal culture, or with clinical signs of Johne's disease. Samples were stored at -18°C and/or -70°C and… (More)
We present the polarization dependence of the ultrafast photoionization for C<sup>+n</sup> (n ≤ 5) fragments from methane. The study extends from the strong field (C<sup>+</sup>, C<sup>+2</sup>) at 10<sup>14</sup> W/cm<sup>2</sup> to the ultrastrong field (C<sup>+5</sup>) at 10<sup>18</sup> W/cm<sup>2</sup>.