Scott J. Strong

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 Differential screening has been used to identify cDNAs encoding a long form of IgX in Raja eglanteria (clearnose skate). Comparisons of the IgX long form with the previously described short-form IgX cDNAs and the genomic IgX locus indicate that the V and two 5′C regions of the short and long forms of IgX are >90% identical at the nucleotide level.(More)
The gene for arginine kinase (AK; EC 2.7.3.3) from the horseshoe crab, Limulus polyphemus, was cloned and the complete cDNA sequence was determined. An open reading frame with 1071 nucleotides was detected that encodes a 357 amino-acid protein with a calculated M(r) of 40,238. The coding transcript is flanked by 13 and 512 nucleotides of 5' and 3'(More)
 Immunoglobulin heavy chain (IgH) genes in representative chondrichthyan fishes (sharks and skates) consist of independently functioning clusters, containing separate variable (V H ), diversity (D H ), and joining (J H ) region elements and constant (C H ) region exons. IgH loci have been characterized in Hydrolagus colliei (spotted ratfish), a modern(More)
Arginine kinase (AK) from the horseshoe crab Limulus polyphemus was expressed in Escherichia coli. The bulk of expressed protein resided in insoluble inclusion bodies. However, approximately 3 mg enzyme protein/l culture was present as active soluble AK. The AK-containing expression vector construct was subjected to site-directed mutagenesis via a(More)
Tec-family tyrosine kinases comprise a family of cytoplasmic kinases that are related to, but are structurally distinct from the Src family of kinases. The Tec-family genes: Btk, Itk, Tec, Txk, and Bmx (Desiderio and Siliciano 1994; Rawlings and Witte 1995) are expressed primarily in hematopoietic lineages. Tec-family kinases contain a pleckstrin homology,(More)
Phosphagen kinases catalyze the reversible transfer of a phosphoryl group between guanidino phosphate compounds and ADP, thereby regenerating ATP during bursts of cellular activity. Large quantities of highly pure arginine kinase (EC 2.7.3.3), the phosphagen kinase present in arthropods, have been isolated from E. coli, into which the cDNA for the horseshoe(More)
As the most phylogenetically distant (from mammals) of the jawed vertebrates, the cartilaginous fish are of considerable importance in terms of our understanding of the immune system evolution. Antigen binding receptors associated with both B(Litman et al. 1985) and T (Rast and Litman 1994; Rast et al. 1995) -cell lineages in higher vertebrates have been(More)
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