Learn More
First prepared in 1831, chloroform was initially employed as an "ideal" anesthetic. Chloroform was later found to cause heart and liver damage and, with the discovery of safer agents, lost importance as a medical anesthetic. Today chloroform is used primarily as a solvent in industry, in the production of antibiotics, as a cleaning agent, and as an(More)
Because of the conflicting data concerning the SARS-CoV inhibitory efficacy of ribavirin, an inosine monophosphate (IMP) dehydrogenase inhibitor, studies were done to evaluate the efficacy of ribavirin and other IMP dehydrogenase inhibitors (5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide (EICAR), mizoribine, and mycophenolic acid) in preventing(More)
Three different RNA carriers were compared for use in microscale RNA isolation and subsequent cDNA synthesis and amplification via the polymerase chain reaction. E.coli rRNA alone gave considerable cDNA synthesis which under standard carrier conditions overwhelmed cDNA synthesis from lymphocyte mRNA. Yeast tRNA caused inhibition of mRNA primed cDNA(More)
Hemorrhagic fever of arenaviral origin is a frequently fatal infectious disease of considerable priority to the biodefense mission. Historically, the treatment of arenaviral infections with alpha interferons has not yielded favorable results. Here we present evidence that interferon alfacon-1, a nonnaturally occurring bioengineered alpha interferon approved(More)
OBJECTIVE To determine the modes of transmission of Aleutian mink disease in a natural outbreak. ANIMALS 5,580 black and 9,087 brown mink from a ranch with an outbreak of Aleutian mink disease. PROCEDURE Each mink had serum tested by counter-electrophoresis for Aleutian disease antibody. If a mink was seropositive for Aleutian disease virus by(More)
Efficient cargo uptake is essential for cell-penetrating peptide (CPP) therapeutics, which deliver widely diverse cargoes by exploiting natural cell processes to penetrate the cell's membranes. Yet most current CPP activity assays are hampered by limitations in assessing uptake, including confounding effects of conjugated fluorophores or ligands, indirect(More)
Fv-4 is a truncated ecotropic retrovirus gene that codes for an envelope protein under control of a cellular promoter. It confers resistance to ecotropic murine leukemia viruses. Transgenic mice were derived using the native Fv-4 gene as the construct for microinjection. Two founder mice were derived. In both founder lines, there was no detectable(More)
  • 1