Sayuri Cocco

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The effect of oral administration of a tryptophan-free amino acid mixture or the same mixture containing tryptophan (Trp) on hippocampal serotonin (5-HT) extracellular levels was studied using in vivo brain microdialysis of freely moving rats. During chloral hydrate anesthesia rats were implanted with dialysis probes in the dorsal hippocampus, and(More)
In this protocol the effect of both an acute and chronic tryptophan (TRP)-free diet on brain serotonin (5-HT) release was studied in rats. Extracellular levels of cortical 5-HT, assessed by in vivo microdialysis, revealed a decrease in the release of this monoamine. Indeed, 120 min after the acute administration of a TRP-free diet, cortical 5-HT release(More)
Vitamin A and its derivatives (retinoids) play important roles in many physiological processes. The recent finding of high levels of cellular retinol-binding protein type 1 immunoreactivity, cellular retinoic acid-binding protein type 1 immunoreactivity and the presence of nuclear retinoid receptors in the central nervous system of adult rodents suggests(More)
It has been hypothesised that rat lines genetically selected for their alcohol preference consume large amounts of ethanol because they have a low 5-HT content. Since brain tryptophan (TRP) availability controls the rate at which neurons synthesise and release serotonin (5-HT), we assessed whether the administration of a TRP-supplemented or TRP-free diet(More)
To assess the activity of septohippocampal cholinergic neurons during the learning of a radial-arm maze task we measured changes in extracellular acetylcholine levels in the hippocampus by means of the vertical microdialysis technique. During the 12 days spent learning the spatial task the extracellular concentration of acetylcholine in the hippocampus was(More)
By using in vivo microdialysis we monitored the extracellular levels of acetylcholine and serotonin in the hippocampus of rats performing a spatial memory task. After rats were trained for 10 consecutive days to master a food-reinforced radial-arm maze task, they were implanted with a microdialysis probe in the dorsal hippocampus. On day 12, rats were(More)
The HCV (hepatitis C virus) Serotyping 1-6 Assay (Murex Laboratories) was evaluated on 303 French HCV-infected patients. Serological typing results were compared to the genotypes obtained from sequence analyses of the 5' noncoding regions of the virus genome from 46 HCV-infected patients, and assay specificity was found to be high (97.6%). The serological(More)
We assessed whether consumption of a diet lacking in tryptophan (TRP) resulted in alteration in learning and memory performance and hippocampal 5-HT release in rats. Two hours after the acute administration of TRP-free (T) and balanced (B) diet rats were trained in a one-trial passive avoidance task. The two groups of rats showed no significant difference(More)
In this study we have defined the molecular basis and correlated the clinical phenotype with the alpha-globin genotype in a large series of patients of Sardinian descent with HbH disease. The most prevalent molecular defect was the deletion of 3 alpha-globin structural genes most commonly the (--/-alpha 3.7) genotype (83.6%) and rarely the (--/-alpha 4.2)(More)
Long-term ethanol consumption in humans and laboratory animals is associated with morphological and functional alterations of brain structures involved in cognitive processes. In the present experiments, we assessed whether voluntary long-term consumption of ethanol by alcohol-preferring (sP) rats under free choice condition with water (also) caused(More)