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Enzymatic Extraction of Pilocarpine from Pilocarpus jaborandi
Pilocarpine is an imidazole alkaloid, found exclusively in the Pilocarpus genus, with huge pharmaceutical importance. In order to extract pilocarpine from Pilocarpus jaborandi, environmentallyExpand
Hydroxylation of long chain fatty acids by CYP147F1, a new cytochrome P450 subfamily protein from Streptomyces peucetius.
Kinetic parameters, including the dissociation constant, Km, NADH consumption assay, production formation rate, and coupling efficiency for CYP147F1 were determined. Expand
Biosynthesis of the nargenicin A1 pyrrole moiety from Nocardia sp. CS682
The evidences are presented that the pyrrole moiety of nargenicin A1 was also derived from l-proline by the coordinated action of three proteins, NgnN4 (proline adenyltransferase),NgnN5 (Proline carrier protein), and Ngn ngnN3 (flavine-dependent acyl-coenzyme A dehydrogenases). Expand
Homology modeling and docking studies of Streptomyces peucetius CYP147F1 as limonene hydroxylase.
Homology modeling of Streptomyces peucetius CYP147F1 was constructed using three cytochrome P450 structures, CYP107L1, CyPVdh, and CYPeryF, as templates to validate the active-site architecture and functionally important residues within the substrate recognition site were identified by subsequent characterization of the secondary structure. Expand
Biotransformation of flavone by CYP105P2 from Streptomyces peucetius.
In a pursuit to hydroxylate the flavone ring, CYP105P2 was co-expressed in a two-vector system with putidaredoxin reductase and putidaringoxin from Pseudomonas putida for efficient electron transport. Expand
Preparation of polyaniline-coated polystyrene nanoparticles for the sorption of silver ions
Abstract Polyaniline-coated polystyrene (PS/PANI) beads with an average diameter of approximately 310 nm were prepared from styrene and divinylbenzene via emulsion polymerization and subsequentExpand
Synthesis of thiourea-immobilized polystyrene nanoparticles and their sorption behavior with respect to silver ions in aqueous phase.
Overall, the novel thiourea-immobilized polystyrene (TA-PS) nanoparticles exhibited a high Ag+ sorption capacity and high selectivity against alkaline and alkaline earth-metal ions, which allows them to be used for long time periods in sorption-desorption processes. Expand
Thiourea-Immobilized Polymer Beads for Sorption of Cr(VI) Ions in Acidic Aqueous Media
Polymer sorbents used for the removal of hexavalent chromium (Cr(VI)) ions from highly acidic aqueous media (pH≤1) are needed. In this study, micronsized polystyrene beads containing immobilizedExpand
In-silico and In-vitro based studies of Streptomyces peucetius CYP107N3 for oleic acid epoxidation
The purified CYP107N3 was shown to catalyze C9-C10 epoxidation of oleic acid in vitro to 9,10-epoxy stearic acid confirmed by ESI-MS, HPLC-MS and GC-MS spectral analysis. Expand