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Fetal bovine serum (FBS) or other mammal-derived factors are extensively supplemented as growth factor into culture medium. Since mammal-derived factors arouses the concern about the risk of zoonosis, serum-and mammal-free culture is strongly required. We focused on sericin hydrolysates, originating from silkworm, and reported that sericin is effective as(More)
The aim of this study is to examine how the amygdala and hippocampus interact for behavioral performance modulated by different Reward-expectations (REs). We simultaneously recorded neuronal spikes and local field potential from the basolateral amygdala and hippocampal CA1 while rats were performing a light-side discrimination task with different(More)
Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This study aims to improve islet(More)
In the present paper, we focus on the coding by cell assemblies in the prefrontal cortex (PFC) and discuss the diversity of the coding, which results in stable and dynamic representations and the processing of various information in that higher brain region. The key activity that reflects cell-assembly coding is the synchrony of the firing of multiple(More)
This research shows a novel method for hazard identification of a chemical and UV light on a single cell level with a laser probe beam. The laser probe beam was passed through interface of cell membrane/culture medium of a cultured human hepatoblastoma cell line HepG2. Deflection of the laser probe beam, which was induced by changes in concentration(More)
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