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Euglena gracilis was found to contain a peroxidase that specifically require L-ascorbic acid as the natural electron donor in the cytosol. The presence of an oxidation-reduction system metabolizing L-ascorbic acid was demonstrated in Euglena cells. Oxidation of L-ascorbic acid by the peroxidase, and the absence of ascorbic acid oxidase activity, suggests(More)
A malonyl-CoA-independent fatty acid synthetic system, different from the systems in other subcellular fractions, occurred in mitochondria of Euglena gracilis. The system had ability to synthesize fatty acids directly from acetyl-CoA as both primer and C2 donor using NADH as an electron donor. Fatty acids were synthesized by reversal of beta-oxidation with(More)
In the assay of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) in vitro, the concentration of CO2, the substrate of the enzyme, has been calculated from the amount of sodium bicarbonate added to the assay mixture with a dissociation constant of carbonic acid in pure water, 6.35 to 6.37. However, Rubisco is generally assayed at ionic strength of(More)
Culture of the green alga Chlamydomonas reinhardtii in the medium containing sodium selenite caused the activity of ascorbate peroxidase to disappear and the appearance of glutathione peroxidase. The induced maximum activity of glutathione peroxidase reached 350 micromole (milligram chlorophyll hour)(-1) under assay conditions used. The enzymic properties(More)
Pyruvate:NADP+ oxidoreductase was homogeneously purified from crude extract of Euglena gracilis. The Mr of the enzyme was estimated to be 309,000 by gel filtration. The enzyme migrated as a single protein band with Mr of 166,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting that the enzyme consists of two identical polypeptides.(More)
We compared shoot and needle morphology and photosynthesis in Picea glehnii (Friedr. Schmidt) M.T. Mast. and Picea jezoensis (Sieb. et Zucc.) Carr. trees planted on immature volcanic ash and well-developed brown forest soils to investigate whether species differences in morphological and physiological plasticity affected tree growth on different soil types.(More)
We directly observe the interaction between 1-butyl-3-methylimidazolium (bmim) or 1-butyl-2,3-dimethylimidazolium (bm(2)im) and the solute, ethyl acrylate (EA), which is the popular dienophile in the Diels-Alder reaction and an H-bonding acceptor, by using specially designed electrospray mass spectrometry. In imidazolium ionic liquids, cation-anion(More)
Following a previous report on physicochemical properties, the enzymological properties of a homogeneously purified preparation of pantothenate synthetase were described. The optimum pH was 10.0 and optimum temperature 30 degrees C. The lyophilized enzyme was very stable on standing at -20 degrees C. K+ or NH4+ and Mg2+ were required as activators; other(More)
Euglena aquacobalamin reductase (NADPH: EC 1.6.99.-) was purified, and its subcellular distribution was studied to elucidate the mechanism of the conversion of hydroxocobalamin to 5'-deoxyadenosylcobalamin. The enzyme was found in the mitochondria. It was purified about 150-fold over the Euglena mitochondrial extract in a yield of 38%. The purified enzyme(More)