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BACKGROUND Rice (Oryza sativa L.) is a highly drought sensitive crop, and most semi dwarf rice varieties suffer severe yield losses from reproductive stage drought stress. The genetic complexity of drought tolerance has deterred the identification of agronomically relevant quantitative trait loci (QTL) that can be deployed to improve rice yield under(More)
A simple, rapid, and efficient method of leaf DNA extraction was optimized for PCR based Marker Assisted Selection (MAS) in rice. The procedure involved homogenization of leaves of 10-days old seedlings in the extraction buffer, followed by centrifugation. The supernatant was directly used for PCR. The isolated DNA had protein and RNA impurities. However,(More)
Drought tolerance in rice is a complex trait collectively determined by numerous component traits. These traits are governed by many genes with huge environmental interactions, with low heritability, and thus are difficult to investigate. In this context, it is important to define the drought tolerance from practical point of view and design a screening(More)
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