Saroja Narasimhan

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Cell suspension culture of critically endangered Coscinium fenestratum was established from young leaf segments on WPM supplemented with auxins. Effect of 2,4-D, IAA, IBA and NAA was examined on cell growth and berberine production. Berberine was synthesized and released continuously into the liquid medium. Presence of 2,4-D stimulated cell growth, but was(More)
Syntheses of chromosomal proteins was studied in relation to DNA syntheses in the rat thymus following whole-body X-irradiation (600 rad). There was a considerable depression of the syntheses of DNA and histones during the first 4--48 hours after irradiation. The syntheses of histones H3 and H4 plus H2A were affected to a much greater degree than those of(More)
The ricin-resistant mutant baby hamster kidney (BHK) cell line RICR21 is unable to make the sialylated bi- or triantennary complexN-glycans found in wild type cells and accumulates instead non-bisected hybrid structures containing three Man residues and one or two sialylated antennae (Hugheset al 1983, Carbohydr Res 120215-34). Specific assays(More)
The enzyme catalyzing this reaction has been named UDP-G1cNAc:glycopeptide 84-N-acetylglucosaminyltransferase I11 (GlcNAc-transferase III) to distinguish it from two other GlcNAc-transferases (I and 11) present in hen oviduct and previously described in several mammalian tissues. GlcNAc-transferases I and 11, respectively, attach GlcNAc in 81-2 linkage to(More)
The carbohydrate-binding specificity of rice (Oryza sativa) lectin was investigated by testing the ability of radioactively labelled glycopeptides and oligosaccharides to bind to a rice lectin-Sepharose 4B column. Rice lectin binds asparagine-linked oligosaccharides through the core NN'-diacetylchitobiose moiety. Whereas beta 1-4-mannose enhances the(More)
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