Sarah Oehlschlager

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This is the report of the fortieth of a series of workshops organised by the European Centre for the Validation of Alternative Methods (ECVAM). ECVAM’s main goal, as defined in 1993 by its Scientific Advisory Committee, is to promote the scientific and regulatory acceptance of alternative methods which are of importance to the biosciences and which reduce,(More)
A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. Multivariate analysis of 1H NMR (nuclear(More)
We describe the use of a model system to mimic chronic toxin exposure, similar to that which might be found in a human situation, where exposure to dietary or environmental toxins occurs at a low level for an extended period of time. This is in contrast to the acute, immediately toxic dose effect usually observed in flask tissue culture. The apparatus used(More)
While many compounds have been reported to change in laboratory based drought-stress experiments, little is known about how such compounds change, and are significant, under field conditions. The Pisum sativum L. (pea) leaf metabolome has been profiled, using 1D and 2D NMR spectroscopy, to monitor the changes induced by drought-stress, under both glasshouse(More)
The aim of this study was to examine the capacity of an in vitro model to test the potential of an allergen to cause cross-linking of IgE bound to the surface of mast cells. The model involved the passive sensitization of murine mast cells, with zearalenone IgE and subsequent exposure to anti IgE, zearalenone, zearalenone-BSA (with up to nine bound(More)
The risks associated with IgE-mediated food allergy highlight the need for methods to screen for potential food allergens. Clinical and immunological tests are available for the diagnosis of food allergy to known food allergens, but this does not extend to the evaluation, or prediction of allergenicity in novel foods. This category, includes foods produced(More)
An interlaboratory study was conducted to evaluate a method for determination of the percentage of RoundUp Ready (RR) soya in soya flour using Taqman technology. The method included DNA extraction from the test portion with cetyltrimethylammonium bromide buffer followed by chloroform extraction and Wizard resin cleanup steps. The DNA was then assayed with(More)
The ScheBo Brainostic test, which detects neuron-specific enolase by Western blotting, and the r-Biopharm Ridascreen Risk Material ELISA test, which detects the presence of glial fibrillary acidic protein, were evaluated using meats containing spinal cord and brain central nervous system (CNS) tissue from ovine and bovine species. The meats were pork,(More)
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