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PURPOSE This study represents a first step toward the evaluation of possible compositional differences in meibum from normal donors (Mn) and donors with meibomian gland dysfunction (Md) by (1)H-NMR spectroscopy. The results highlight the applicability of (1)H-NMR spectroscopy for the quantitative analysis of waxes, cholesteryl esters, and glycerides in(More)
PURPOSE Human tear film stability decreases with increasing age. In this study, the changes in meibum composition were measured in search of markers of tear film instability. METHODS (1)H NMR nuclear magnetic resonance (NMR) spectra of 43 normal donors aged 1 to 88 years were acquired. RESULTS Compared with meibum from adolescents and adults, meibum(More)
PURPOSE Nuclear magnetic resonance (NMR) spectroscopy has been used to quantify lipid wax, cholesterol ester terpenoid and glyceride composition, saturation, oxidation, and CH₂ and CH₃ moiety distribution. This tool was used to measure changes in human meibum composition with meibomian gland dysfunction (MGD). METHODS (1)H-NMR spectra of meibum from 39(More)
1H-NMR spectroscopy has been used to quantify squalene in meibum and sebum. Squalene has many beneficial properties and its loss on the surface of skin upon ultraviolet light exposure or in the tear film with dry eye could be detrimental. In this study, we confirm the NMR proton resonance assignments of squalene, squalene in human meibum, and in human(More)
Recent NMR studies suggest that unsaturation may contribute to tear film instability in adults and loss of cholesteryl esters and squalene could reduce tear film stability in adults with meibomian gland dysfunction. The proton resonances were tentatively assigned in those studies. In this current investigation, meibum from seven infants and children, one(More)
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