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The glycosylation of the flagellin of the polar flagellum of AzospirMum brssilense Sp7 is demonstrated in several ways: (1) by a decrease in apparent M, after chemical deglycosylation; (2) by sugar staining after SDS-PAGE; (3) by use of a sugar-specif ic monoclonal antibody in immunogold labelling coupled with transmission electron microscopy. In addition,(More)
Monoclonal antibodies (mAbs) with high specificity for Azospirillum brasilense Sp7, and which bind to different antigenic determinants, were characterized using Western blot techniques applied to one-and two-dimensional fingerprints of the outer-membrane components, and by immunogold labelling combined with transmission electron microscopy. One class of(More)
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