Sara Farrah Heuss

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Genetic studies have shown that ubiquitination and endocytosis of the Drosophila ligand Delta in signal-sending cells are required for activation of Notch signaling, but how these events promote Notch activation remains poorly understood. Here, we show that an ubiquitination-defective mutant of the murine Delta-homologue Dll1 is endocytosed but, in contrast(More)
Activation of the mammalian Notch receptor after ligand binding relies on a succession of events including metalloprotease-cleavage, endocytosis, monoubiquitination, and eventually processing by the gamma-secretase, giving rise to a soluble, transcriptionally active molecule. The Notch1 receptor was proposed to be monoubiquitinated before its(More)
The Notch pathway is involved in cell-cell signaling during development and adulthood from invertebrates to higher eukaryotes. Activation of the Notch receptor by its ligands relies upon a multi-step processing. The extracellular part of the receptor is removed by a metalloprotease of the ADAM family and the remaining fragment is cleaved within its(More)
The activity of Notch ligands is tightly regulated by trafficking events occurring both before and after ligand-receptor interaction. In particular endocytosis and recycling have been shown to be required for full signaling activity of the ligands before they encounter the Notch receptor. However little is known about the precise endocytic processes that(More)
Unité de Signalisation Moléculaire et Activation Cellulaire, URA CNRS 2582, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France Fax: 33 1 40 61 30 40 Unité du Dévelopement Normal et Pathologique du Système Immunitaire, Unité INSERM 768, Groupe Hospitalier Necker-Enfants Malades, 149 rue de Sèvres, 75743 Paris Cedex 15, France(More)
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