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IMPORTANCE Physical rehabilitation in the intensive care unit (ICU) may improve the outcomes of patients with acute respiratory failure. OBJECTIVE To compare standardized rehabilitation therapy (SRT) to usual ICU care in acute respiratory failure. DESIGN, SETTING, AND PARTICIPANTS Single-center, randomized clinical trial at Wake Forest Baptist Medical(More)
Recent studies reporting differentiation of early neural progenitors of human adipose tissue-derived stromal cells (ADSCs) has aroused interest among investigators for regenerative medicine. The aim of this study was to investigate the differentiation of ADSCs to neuron-like cells and to extend the life span of these differentiated ADSCs in vitro using our(More)
Our studies on the control of extracellular protease synthesis in an Aspergillus flavus strain isolated by us indicate that in a defined medium a protein must be present for enzyme to be produced. Soya bean protein and cotton seed protein were efficient inducers. The ability to induce enzyme synthesis was a characteristic property of the individual protein.(More)
Recent studies suggest that human adipose tissue contains pluripotent stem cells, which are similar to bone marrow-derived stem cells. The objective of the present study was to assess the effect in bone regenerating capability of human adipose-derived stem cells (ADSCs) cultured in osteogenic media layered over poly lactide-co-glycolic acid (PLGA) and(More)
The osteogenic potential of mesenchymal stem cells (MSCs) cultured on poly(lactide-co-glycolide) (PLGA) or poly(caprolactone) (PCL), two widely used polymeric biomaterials that have been reported to differentially support osteogenic differentiation, was compared in these studies. Here we report that MSCs cultured in 3-D PLGA scaffolds for up to 5 weeks(More)
The physico-chemical properties of the purified acid proteinase were investigated. This enzyme was found to hydrolyze hemoglobin maximally among the different substrates used. The maximum hydrolysis of hemoglobin was found at pH 2.8 and 45 degrees C in 30 min. Km value of 5.5 X 10(-4) M was obtained from the Lineweaver Burk plot for the hydrolysis of(More)
A 24-year-old man developed cyclic esotropia following vitreoretinal surgery for retinal detachment in his right eye. He underwent right eye medial rectus recession (6 mm) and lateral rectus resection (8.5 mm) under local anesthesia for the correction of cyclic esotropia. Following surgery, he developed a unique pattern of cyclic strabismus that involved(More)
The effect of new non-steroidal anti-inflammatory agents on lysosomal stability was studied by determining the activity of beta-glucuronidase, a typical lysosomal enzyme, in various sub-cellular fractions and its release from the lysosome-rich fraction. Adjuvant arthritic animals showed a significant increase in the beta-glucuronidase activity in(More)
Tissue-engineered constructs offer a new hope to patients suffering from functional impairment after nerve injury. An effort has been made to focus on delivery, regulation, and "molecular shutoff" of nerve growth factor (NGF) in tissue-engineered constructs. We have previously demonstrated that human embryonic kidney (HEK-293) cells can be genetically(More)
The extracellular acid proteinase from A. fumigatus was purified by employing several chromatographic procedures and a 164 fold purification with 22% yield was achieved. The homogeneity of the enzyme was confirmed by SDS-Polyacrylamide gel electrophoresis, immodiffusion and immunoelectrophoresis techniques.