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In recent years the intake of n-6 PUFA and trans-fatty acids (TFA) has increased, whereas n-3 PUFA intake has decreased. The present study investigated the effects of maternal diet high in n-6 PUFA, n-3 PUFA or TFA on glucose metabolism, insulin sensitivity and fatty acid profile in male offspring. Female weanling Wistar/NIN rats were randomly assigned to(More)
A detection method for genetically modified foods is an essential regulatory requirement for many countries. The present study is aimed at developing a qualitative method for detection of genetically modified organisms by combining PCR methodology with capillary gel electrophoresis (PCR-CGE) in a sequencing platform to detect Bacillus thuringiensis(More)
Lipoxygenase (Lox) has been extensively purified from aromatic (Bas-370) and non-aromatic (Pusa-834) rice varieties. Crude isolates of Lox from the aromatic varieties (Bas-370 and PB-1) showed higher specific activity (4-fold) when compared to non-aromatic varieties (Pusa-677 and Pusa-834). The activity was optimum at pH 8.0 in all four varieties. Anionic(More)
An efficient PCR-based method to trace genetically modified food and feed products is in demand due to regulatory requirements and contaminant issues in India. However, post-PCR detection with conventional methods has limited sensitivity in amplicon separation that is crucial in multiplexing. The study aimed to develop a sensitive post-PCR detection method(More)
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