Sangeeta Saxena

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Indole acetic acid (IAA/auxin) profoundly affects wood formation but the molecular mechanism of auxin action in this process remains poorly understood. We have cloned cDNAs for eight members of the Aux/IAA gene family from hybrid aspen (Populus tremula L. x Populus tremuloides Michx.) that encode potential mediators of the auxin signal transduction pathway.(More)
The cambium of woody plants cycles between active and dormant states. Dormancy can be subdivided into eco- and endodormant stages. Ecodormant trees resume growth upon exposure to growth-promotive signals, while the establishment of endodormant state results in loss of the ability to respond to these signals. In this paper, we analysed the regulation of(More)
MicroRNAs are small non-coding RNAs that regulate gene expression at multiple levels. The discovery of virally encoded miRNAs attracted immense attention towards their role in viral replication and pathogenesis. Kaposi's-sarcoma-associated herpes virus encodes miRNA that functions as an orthologue of human cellular miRNA, i.e., hsa-miR-155. Keeping the same(More)
Use of siRNA is a powerful methodology to particularly knockdown the targeted genes in a sequence specific manner. The potential of siRNA can be harnessed for silencing specific geminiviral genes in papaya and tomato plant hosts, thus making them resistant to the respective viruses. The challenge is in designing exogenous siRNA which can trigger silencing(More)
The studies into the pathophysiology of viral miRNAs are still in infancy; the interspecies regulation at the miRNA level fuels the spark of the investigation into the repertoire of virus–host interactions. Reports pertaining to the viral miRNAs role in modulating/evading the host immune response are surging up; we initiated this in silico study to(More)
Geminiviruses are single-stranded circular DNA viruses causing leaf curl disease in papaya crop. Post-Transcriptional Gene Silencing (PTGS), also known as RNAi, acts as a natural antiviral defence mechanism and plays a role in genome maintenance and development in plants. PTGS suppression by viruses makes the plant RNA silencing machinery inefficient. Three(More)
Centella asiatica (L), a herbaceous plant belonging to the family Apiaceae, possesses great medicinal value owing to the presence of important and characteristic triterpenoids as secondary metabolites. These triterpenoid secondary metabolites are found in leaves in substantial quantities whereas negligible amounts may be detected sometimes in root tissues.(More)
An efficient, simple and commercially applicable protocol for rapid micropropagation of sugarcane has been designed using variety Co 05011. Pretreatment of shoot tip explants with thidiazuron (TDZ) induced high frequency regeneration of shoot cultures with improved multiplication ratio. The highest frequency (80%) of shoot initiation in explants pretreated(More)
Three alkaline protease producing gram positive, spore forming Streptomyces (J1, J2 and J3) were isolated from alkaline soil (pH range: 9-10) samples collected from Central Soil Salinity Research Institute (C.S.S.R.I), Lucknow. On the basis of halo zone, morphology, texture and microscopic characteristics they were identified as Streptomyces. Further(More)
Micro RNAs (miRNAs) are a class of 21-24 nucleotide long single stranded, endogenous, noncoding RNAs involved mainly in post transcriptional gene silencing in animals and plants. Their role in the gene regulation of plant growth and development, biotic and abiotic stress responses, methylation is revealed and much more is expected by these tiny molecules.(More)