Mycelial growth and survival ratio of ectomycorrhizal fungi were determined after storage at -70℃ for 1, 3, or 6 mon. Seventeen of 23 ectomycorrhizal fungi did not survive after storage for more than 6 mon, whereas Cenococcum geophilum, Lepista nuda, and some species of Rhizopogon and Suillus did survive.
INTRODUCTION Angiogenesis plays a critical role in synovial inflammation and joint destruction in rheumatoid arthritis (RA). Vascular endothelial growth factor A (VEGF-A) and angiopoietins are two important mediators of synovial angiogenesis. We have previously developed a novel chimeric decoy receptor, namely, double-antiangiogenic protein (DAAP), which… (More)
We studied the resistance of Cenococcum geophilum and Suillus granulatus isolates to NaCl during growth under axenic culture conditions. C. geophilum isolates displayed variations in NaCl resistance; mycelial growth of most isolates was inhibited above 200mM. All isolates of S. granulatus were tolerant to high NaCl content.
To assess the regional difference and influence of the biological variables on atherosclerosis in female, we analyzed 7 segments of aorta (2 ascending, 3 thoracic, and 2 abdominal) from 90 superficially healthy Korean women (39+/-14 yr of age) who died from external causes. Tissue specimens were macroscopically examined and histopathologically divided into… (More)
OBJECTIVE To determine the utility of intercellular adhesion molecule (ICAM)-1 antibody-conjugated gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA-anti-ICAM-1) as a targeted contrast agent for the molecular magnetic resonance imaging (MRI) in collagen-induced arthritis (CIA). MATERIALS AND METHODS THREE GROUPS OF MICE WERE USED: non-arthritic… (More)
Enzyme activities of Cenococcum geophilum isolates were examined on enzyme-specific solid media. Deoxyribonuclease, phosphatase, and urease were detected in all isolates, whereas cellulase was not detected in any of the isolates. Variations in enzyme activities of amylase, caseinolysis, gelatinase, lipase, and ribonuclease were observed among isolates.
Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial identification. In this study, we applied SCAR markers for the rapid and accurate detection of Phytophthora katsurae, the casual agent of chestnut ink disease in Korea. In this study, we developed seven SCAR markers specific to P. katsurae… (More)
We designed two specific primer pairs, KatI 3F/KatI 5R and KatI 4F/KatI 5R to detect P. katsurae by PCR and each primer produced specific amplicons of 747 bp and 721 bp, respectively. To assess the specificity and sensitivity of primer, 10-fold serial dilutions of P. katsurae genomic DNA were made ranging in concentration from 1 mgml−1 to 1 pgml−1. Using… (More)
We investigated the ectomycorrhizal (EcM) fungal community of naturally regenerating Pinus densiflora seedlings on exposed decomposed granite (DG) slopes along woodland paths in the Republic of Korea. We sampled three- to five-year-old seedlings from slopes where DG was exposed (habitat S) and from the surrounding forest edge or gaps (habitat F). We… (More)