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Subgenomic flavivirus RNA binds the mosquito DEAD/H-box helicase ME31B and determines Zika virus transmission by Aedes aegypti
TLDR
Comparison of mosquito infection via infectious blood meals and intrathoracic injections showed that sfRNA is important for ZIKV to overcome the mosquito midgut barrier and to promote virus accumulation in the saliva, and mechanistic insights into the functions of this noncoding RNA are provided.
The invasive Asian bush mosquito Aedes japonicus found in the Netherlands can experimentally transmit Zika virus and Usutu virus
Background The Asian bush mosquito Aedes japonicus is invading Europe and was first discovered in Lelystad, the Netherlands in 2013, where it has established a permanent population. In this study, we
Competition between Usutu virus and West Nile virus during simultaneous and sequential infection of Culex pipiens mosquitoes
TLDR
It is shown that USUV is outcompeted by WNV in mammalian, avian and mosquito cells during co-infection, which indicates that the chance of concurrent USUV and WNV transmission via a single mosquito bite is low.
Relocation of the attTn7 Transgene Insertion Site in Bacmid DNA Enhances Baculovirus Genome Stability and Recombinant Protein Expression in Insect Cells
TLDR
It is concluded that GOI expression from the odv-e56 (pif-5) locus results in higher overall expression levels and is more stable over serial passages compared to the original bacmid, thereby resulting in higher and prolonged recombinant protein expression levels.
Forced Zika Virus Infection of Culex pipiens Leads to Limited Virus Accumulation in Mosquito Saliva
TLDR
A strong midgut barrier restricted virus dissemination in the mosquito after oral exposure and the common house mosquito Cx.
Effect of blood source on vector competence of Culex pipiens biotypes for Usutu virus
TLDR
Interestingly, biotype molestus is less likely to transmit USUV compared to biotype pipiens due to very low virus titers in the saliva, and the blood source had no effect on the vector competence for USUV.