Sandra J. Spurr

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Intact, viable sheets of adult rabbit corneal epithelium, 9 mm in diameter, were prepared by the Dispase II method (Gipson, I. K., and S. M. Grill, 1982, Invest. Ophthalmol. Vis. Sci. 23:269-273). The sheets, freed of the basal lamina, retained their desmosomes and stratified epithelial characteristics, but lacked hemidesmosomes (HD). Epithelial sheets were(More)
An ultrastructural and immunohistochemical comparison was made between the effects of Dispase II and EDTA on the basement membrane zone of corneal epithelium. The comparison was made on intact corneas as well as on freed epithelial sheets and remnant stromas that had been separated using either the enzyme or chelator. At the ultrastructural level,(More)
Sheets of corneal epithelium removed from 9-mm buttons of adult rabbit corneas using Dispase II were placed on abraded (basement membrane intact) or keratectomized corneas of anesthetized rabbits. Both types of wounds extended from limbus to limbus. The host animals were maintained under deep anesthesia for 3 hr, during which time culture medium was dripped(More)
Recently, we developed a procedure for removing large, intact, viable sheets of corneal epithelium from adult rabbit and rat corneas using the bacterial neutral protease, Dispase II. 1 These sheets have been used for developing an in vitro system to study hemides-mosome (HD) formation, 23 as a source of "pure" epithelium for tissue culture, 4 and for(More)
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