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Eukaryotic cells control the initiation of DNA replication so that origins that have fired once in S phase do not fire a second time within the same cell cycle. Failure to exert this control leads to genetic instability. Here we investigate how rereplication is prevented in normal mammalian cells and how these mechanisms might be overcome during tumor(More)
A 21-base pair RNA duplex that perfectly matches an endogenous target mRNA selectively degrades the mRNA and suppresses gene expression in mammalian tissue culture cells. A single base mismatch with the target is believed to protect the mRNA from degradation, making this type of interference highly specific to the targeted gene. A short RNA with mismatches(More)
Anxiety and depression are major chronic mood disorders, and the etiopathology for each appears to be repeated exposure to diverse unpredictable stress factors. Most of the studies on anxiety and related mood disorders are performed in rodents, and a good model is chronic unpredictable stress (CUS). In this study, we have attempted to understand the(More)
Geminin is a cellular protein that associates with Cdt1 and inhibits Mcm2-7 loading during S phase. It prevents multiple cycles of replication per cell cycle and prevents episome replication. It also directly inhibits the HoxA11 transcription factor. Here we report that geminin forms a parallel coiled-coil homodimer with atypical residues in the dimer(More)
When mammalian cells experience radiation insult, DNA replication is stalled to prevent erroneous DNA synthesis. UV-irradiation triggers proteolysis of Mcm10, an essential human replication factor, inhibiting the ongoing replication. Here, we report that Mcm10 associates with E3 ubiquitin ligase comprising DNA damage-binding protein, DDB1, cullin, Cul4 and(More)
Uveitis is an inflammation of the uveal tract and is one of the major causes of visual impairment. Several lines of evidence suggest an important role for activated T lymphocytes in the perpetuation of posterior uveitis. In sequel to our preliminary observations with human S-antigen, we have further investigated the proliferative response of peripheral(More)
97 children, 67 girls aged 0-13 years and 30 boys aged 0-11 years, with apparent first urinary-tract infections were investigated radiologically. The intravenous pyelogram was abnormal in 15.5% of the girls and 33% of the boys and the micturating cystourethrogram was abnormal in 34% of the girls and 39% of the boys. When both examinations were combined the(More)
Astrocytic hamartomas are rare benign tumours. Spectral domain optical coherence tomography (SD-OCT) with advanced visualisation (optic disc cube 200×200) was performed on a 27-year-old man with peripapillary astrocytic hamartomas in the right eye. En face (OCT scan) view (CT scan) of the optic nerve at the level of retinal pigment epithelium revealed optic(More)
PURPOSE To evaluate the efficacy of topical bevacizumab for corneal neovascularization in graft rejection. METHODS A 55-year-old man presented with corneal neovascularization and graft rejection 6 months following penetrating keratoplasty (triple procedure) in the left eye. His best-corrected visual acuity (BCVA) was counting fingers at 1 meter. He was(More)