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The Neisseria gonorrhoeae type IV secretion system secretes chromosomal DNA that acts in natural transformation. To examine the mechanism of DNA processing for secretion, we made mutations in the putative relaxase gene traI and used nucleases to characterize the secreted DNA. The nuclease experiments demonstrated that the secreted DNA is single-stranded and(More)
A vital function of the cell membrane in all living organism is to maintain the membrane permeability barrier and fluidity. The composition of the phospholipid bilayer is distinct in archaea when compared to bacteria and eukarya. In archaea, isoprenoid hydrocarbon side chains are linked via an ether bond to the sn-glycerol-1-phosphate backbone. In bacteria(More)
BACKGROUND Most strains of Neisseria gonorrhoeae carry a Gonococcal Genetic Island which encodes a type IV secretion system involved in the secretion of ssDNA. We characterize the GGI-encoded ssDNA binding protein, SsbB. Close homologs of SsbB are located within a conserved genetic cluster found in genetic islands of different proteobacteria. This cluster(More)
Structures of the type IV pili secretin complexes from Neisseria gonorrhoeae and Neisseria meningitidis, embedded in outer membranes were investigated by transmission electron microscopy. Single particle averaging revealed additional domains not observed previously. Secretin complexes of N. gonorrhoeae showed a double ring structure with a 14-15-fold(More)
— In order to suppress the Multiple-Access Interference (MAI) in the DS CDMA system, a new RAKE receiver based on parallel interference elimination is proposed in this paper. The multiple-access interference is evaluated by tentative decision and known user information. Then the performance over Rayleigh fading channel are analyzed and compared to MRC(More)
With the world turning to be a " Global-village " , everything available to us is in floods but as there are opportunities there are even the challenges that rule the order. The Globally Distributed Work actually creates a different environment in terms of Play-rules, Play-Platforms and Play-Competitors thus posing a need to have an insight through the lens(More)
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