Samin Hong

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BACKGROUND Agmatine is an endogenous polyamine formed by the decarboxylation of L-arginine. We investigated the protective effects of agmatine against hypoxia-induced apoptosis of immortalized rat retinal ganglion cells (RGC-5). RGC-5 cells were cultured in a closed hypoxic chamber (5% O2) with or without agmatine. Cell viability was determined by lactate(More)
To better understand the neuroprotective actions of agmatine in ischemic insults, its effects on astrocytes were investigated using an in vitro oxygen-glucose deprivation (OGD) model. After primary culture, cortical astrocytes were moved into a closed anaerobic chamber and incubated in glucose-free culture media. 4 h later, the cells were restored to(More)
PURPOSE To establish an effective system for isolating primary retinal ganglion cells (RGCs) from newborn mice. METHODS The retinas were separated from enucleated eyeballs of Crl:CD-1 mice on postnatal day 1 to 4. RGCs were purified using three different methods, including two-step immunopanning (TSI), direct magnetic separation (DMS), and(More)
PURPOSE To verify the effect of the scan angle of the optic nerve head (ONH) on measurements of peripapillary retinal nerve fiber layer (RNFL) thickness by using spectral-domain optical coherence tomography (OCT). METHODS Both eyes of 64 healthy volunteers were scanned by the optic disc cube 200 x 200 scan of a spectral-domain OCT system. Ultra-high(More)
Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine,(More)
PURPOSE To assess the reproducibility and agreement of anterior chamber measurements between the Pentacam (PTC) and the Anterior segment optical coherence tomography (AOCT) in normal healthy eyes with open angle. METHODS Prospective cross-sectional comparative case series. A total of 162 eyes of 81 healthy volunteers with normal open angle were included(More)
PURPOSE To classify medically treated open-angle glaucoma (OAG) by the pattern of progression using hierarchical cluster analysis, and to determine OAG progression characteristics by comparing clusters. METHODS Ninety-five eyes of 95 OAG patients who received medical treatment, and who had undergone visual field (VF) testing at least once per year for 5(More)
Microglia are the resident macrophages of CNS and play a crucial role in maintaining homeostasis against various neuronal injuries. However, excessive activation of microglia may destroy healthy neurons as well as damaged neurons. We investigated neuroprotective effects of amgatine on hypoxic microglia using in vitro and in vivo models for transient(More)
BACKGROUND Humphrey Matrix perimetry is a recent development in automated perimetry that uses frequency-doubling technology (FDT). We evaluated the learning effect of Humphrey Matrix perimetry with a full-threshold 30-2 strategy. METHODS Twenty-four patients with primary open-angle glaucoma and early visual field defects as assessed by standard automated(More)
PURPOSE To gain a better understanding of the roles of interleukins (ILs) in subconjunctival fibrosis, we investigated their expression in transforming growth factor-beta1 (TGF-beta1)-stimulated Tenon's fibroblasts and examined their association with the transdifferentiation of fibroblasts to myofibroblasts. METHODS After primary culture, fibroblasts(More)