Samantha Gallagher

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Considerable knowledge regarding skeletal muscle physiology and disease has been gleaned from cultured myoblastic cell lines or isolated primary myoblasts. Such muscle cultures can be induced to differentiate into multinucleated myotubes that become striated. However they in general do not fully mature and therefore do not model mature muscle.(More)
Polyclonal antibodies raised against the tonoplast and plasma membrane H+-ATPases of corn were used to probe Western blots lof clathrin-coated vesicles purified from zucchini hypocotyls and bovine brain. Antibodies to two tonoplast H+-ATPase subunits recognized their respective polypeptides in purified zucchini coated vesicle preparations. Antibody raised(More)
The aim of this study was to describe and analyze the regulation and spatio-temporal dynamics of melanocyte migration in vitro and its coupling to cell division and interaction with the matrix. The melanocyte lineage is particularly interesting because it is involved in both embryonic development and oncogenesis/metastasis (melanoma). Biological experiments(More)
Immunoblotting (often referred to as western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. Following electrophoretic separation of proteins and transfer from the gel to an appropriate membrane, the immobilized proteins are probed with specific antibodies to identify and quantitate any antigens present.(More)
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