Salvatore Orlando

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Gene knockout is the most powerful tool for determining gene function or permanently modifying the phenotypic characteristics of a cell. Existing methods for gene disruption are limited by their efficiency, time to completion, and/or the potential for confounding off-target effects. Here, we demonstrate a rapid single-step approach to targeted gene knockout(More)
We previously demonstrated high-frequency, targeted DNA addition mediated by the homology-directed DNA repair pathway. This method uses a zinc-finger nuclease (ZFN) to create a site-specific double-strand break (DSB) that facilitates copying of genetic information into the chromosome from an exogenous donor molecule. Such donors typically contain two(More)
Samples of the lichen Pseudevernia furfuracea (L.) Zopf and the moss Hypnum cupressiforme Hedw. were exposed for 6 weeks in nylon bags in two air pollution monitoring stations in Trieste and Naples (Italy) with different climates and pollution loads to evaluate influence of environmental conditions on sample vitality. This was assessed before and after(More)
This paper presents a failure analysis of the Java Virtual Machine providing useful insights into the nature of reported failures and to improve the understanding of its dependability aspects. Failure data is extracted from publicly available bug databases, where developers and users of Java applications usually submit failures/bugs. Presented results(More)
We conducted a randomized, double-blind, placebo-controlled study to evaluate the efficacy of oral trimethoprim/sulfamethoxazole (TMP/SMX) in the prevention of bacterial infections in children with cancer. Sixty-three patients with acute leukemia were studied during the induction phase of chemotherapy; 28 patients with solid tumors who were starting(More)
A monitoring infrastructure is a key component in each task aimed at evaluating the dependability of a system. This paper presents a monitoring infrastructure for the Java Virtual Machine (JVM), which is starting to be employed in mission and safety critical application, often with real-time requirements. This infrastructure, named JVMMon, collects data(More)
In this work we investigate software aging phenomena inside the Java Virtual Machine (JVM). Starting from an experimental campaign on real world testbeds, this work isolates the contribution of the JVM to the overall aging trend, and identifies, through statistical methods, which workload parameters are more relevant to aging dynamics. Experimental results(More)
Conventional tangential flow filtration (TFF) has traditionally been limited to separation of solutes that differ by about ten-fold in size. Wide pore-size distributions, membrane fouling, and concentration polarization phenomena have commonly been cited as reasons for this limitation. The use of TFF in the biotechnology industry has therefore been(More)