Sahar F. Mahmoud

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Slo gene-encoded BK potassium channels are prominent in both adrenomedullary and pituitary tissues. At one alternative splicing site, both tissues express variants with and without the optional 'STREX' exon. In adrenal chromaffin cells, this splicing choice, which has important ramifications for cell excitability, has been shown to be regulated by steroid(More)
It was previously hypothesized that stress hormones regulate the alternative splicing of Slo potassium channels, thereby tuning the intrinsic excitability of adrenal chromaffin cells. Male tree shrews subjected to chronic stress by exposure to a dominant male develop robust symptoms with parallels to human depression. We report here that adrenals from males(More)
Much interest has been shown in the use of multi-template reverse transcription-polymerase chain reaction (RT-PCR) as a quantitative instrument for low-abundance mRNAs. A desire to achieve finely-graded quantification of the stress- and hormone-related regulation of one splicing decision in an ion channel gene motivated us to test the reliability of(More)
We have previously shown that store-associated microdomains of high Ca(2+) are not essential for exocytosis in RBL-2H3 mucosal mast cells. We have now examined whether Ca(2+) microdomains near the plasma membrane are required, by comparing the secretory responses seen when Ca(2+) influx was elicited by two very different mechanisms. In the first, antigen(More)
Effect of replacing milk fat with Littesse ® (a carbohydrate based fat repalcer) on the quality of ice milk was studied. 25, 50, 75 and 100% of milk fat was replaced by the same amount of Littesse ® . Substitution of milk fat with Littesse ® caused a significant increase in specific gravity, weight per gallon and titratable acidity, while decreased the(More)
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