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One hundred (100) bovine bulk milk samples were tested for the presence of Coxiella burnetii and the detection of associated risk factors. Of the tested samples, 11% tested positive for C. burnetii. There was no correlation between the presence of C. burnetii in bulk milk samples and the kind of dairy herd the samples were taken from the number of cows in(More)
Helicobacter pylori gram-negative bacteria commonly infect the human gastrointestinal (GI) tract and are readily diagnosed by endoscopy. H. pylori infection causes a broad range of host symptoms from discomfort to significant GI disorders (GIDs). Severity of the clinical manifestations depends mainly upon bacterial load. In this cross-sectional study, we(More)
Helicobacter pylori infection can be eradicated in up to 90 % of patients using current combination of triple therapies, of which clarithromycin is a key component. The development of clarithromycin resistance in H. pylori is recognized as a significant contributing factor in treatment failure. The aim of this study is to compare two fast and direct(More)
BACKGROUND The emergence of antimicrobial resistant strains of Escherichia coli has raised considerable interest in understanding the diversity and epidemiology of E. coli infections in humans. Virulence factors of E. coli determine the specific infections caused by this microorganism. OBJECTIVES This study aimed to determine the prevalence of eight E.(More)
Hepatitis C virus (HCV) is a major public health problem and a leading cause of chronic liver disease. An estimated 180 million people are infected worldwide. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection of HCV genomic RNA and compared the sensitivity of LAMP with nested-PCR. A total of 30 blood(More)
Resistance to oxyimino cephalosporins antibiotics in Enterobacteriaceae is primarily done by the extended spectrum β-lactamases (ESBLs). Clear identification of risk factors for ESBLs-producing infections is necessary. Therefore, efficient strategies can be developed to decrease outbreak of these infections. The aim of this study was to determine the(More)
BACKGROUND AND OBJECTIVE Diarrheal disease is still a major health problem, especially in developing countries, where it is considered as one of the leading causes of morbidity and mortality especially in children. Studies showed that Diarrheagenic E. coli (DEC) such as STES and EPEC strains are among the most prevalent causative agents in acute diarrhea,(More)
CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine phosphorylation motifs (TPMs) of H. pylori. A set of 302 H.(More)
Many different methods have been presented by many researchers for characterizing cagA phosphorelation mothif, and based on different goals, these methods have been used in different studies. For example: PCR-RFLP and real-time PCR methods were presented by Owen et al. (2003), and different PCR methods were presented by Argent et al. (2005), Batista et al.(More)
BACKGROUND β-lactam resistance is more prevalent in Gram negative bacterial isolates worldwide, particularly in developing countries. In order to provide data relating to antibiotic therapy and resistance control, routine monitoring of corresponding antibiotic resistance genes is necessary. AIMS The aim of this study was the characterization of β-lactam(More)
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