Sabrina N. Powell

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[13C6]salicylate, [U-13C]naphthalene, and [U-13C]phenanthrene were synthesized and separately added to slurry from a bench-scale, aerobic bioreactor used to treat soil contaminated with polycyclic aromatic hydrocarbons. Incubations were performed for either 2 days (salicylate, naphthalene) or 7 days (naphthalene, phenanthrene). Total DNA was extracted from(More)
We investigated enrichment with salicylate as a method to stimulate the degradation of polycyclic aromatic hydrocarbons (PAHs) by a microbial communityfrom a bioreactortreating PAH-contaminated soil. DNA-based stable isotope probing (SIP) was used to compare the effect of alternate methods of salicylate addition (spike vs slow, continuous addition) on the(More)
To determine whether the diversity of pyrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon-contaminated soil is affected by the addition of inorganic nutrients or by slurrying the soil, various incubation conditions (all including phosphate buffer) were examined by mineralization studies and stable-isotope probing (SIP). The addition of(More)
Stable-isotope probing (SIP) has been used to determine which microorganisms in a complex environmental sample are capable of metabolizing a labeled substrate. We hypothesized that DNA-based stable-isotope probing with a combination of a (13)C-labeled carbon source and a second, unlabeled carbon source could be combined with analyses of the entire gradient(More)
Plant disease resistance (R) genes control the plant immune response upon pathogen recognition. R proteins initiate cellular events that efficiently limit pathogen reproduction. Most plant R proteins defined to date possess a central nucleotide binding (NB) domain together with C-terminal leucine rich repeats (LRR), and are hence termed NB-LRR proteins.(More)
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