Sabine K. Schmitz

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The shape, structure and connectivity of nerve cells are important aspects of neuronal function. Genetic and epigenetic factors that alter neuronal morphology or synaptic localization of pre- and post-synaptic proteins contribute significantly to neuronal output and may underlie clinical states. To assess the impact of individual genes and disease-causing(More)
The presynaptic active zone mediates synaptic vesicle exocytosis, and modulation of its molecular composition is important for many types of synaptic plasticity. Here, we identify synaptic scaffold protein liprin-α2 as a key organizer in this process. We show that liprin-α2 levels were regulated by synaptic activity and the ubiquitin-proteasome system.(More)
Presynaptic cannabinoid (CB1R) and metabotropic glutamate receptors (mGluR2/3) regulate synaptic strength by inhibiting secretion. Here, we reveal a presynaptic inhibitory pathway activated by extracellular signal-regulated kinase (ERK) that mediates CB1R- and mGluR2/3-induced secretion inhibition. This pathway is triggered by a variety of events, from foot(More)
Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely unknown. We quantified activity-dependent DCV release in hippocampal neurons at single vesicle resolution. DCVs fused preferentially at synaptic terminals. DCVs also fused at extrasynaptic sites but(More)
Different regulatory principles influence synaptic coupling between neurons, including positional principles. In dendrites of pyramidal neurons, postsynaptic sensitivity depends on synapse location, with distal synapses having the highest gain. In this paper, we investigate whether similar rules exist for presynaptic terminals in mixed networks of pyramidal(More)
in preparation 96 4 Abstract Cyclin-dependent kinase 5 (Cdk5) functions in many neuronal processes and has recently been implicated in homeostatic plasticity. Cdk5 activation results in reallocation of synaptic strength, including weakening of some connections while strengthening others. The molecular mechanisms underlying these phenomena are thus far not(More)
Presynaptic inhibition upon CB1 and mGluR2/3 receptor activation requires ERK/MAPK phosphorylation of Munc18-1 Sabine K. Schmitz, Cillian King, Christian Kortleven, Vincent Huson, Tim Kroon, Josta T. Kevenaar, Desiree Schut, Ingrid Saarloos, Joost P. Hoetjes, Heidi de Wit, Oliver Stiedl, Sabine Spijker, Ka Wan Li, Huibert D. Mansvelder, August B. Smit,(More)
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