SUSUMU HORIKAWA

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Penicillin-binding proteins of three Streptomyces strains, S. cacaoi, S. olivaceus, and S. clavuligerus, were examined by gel electrophoresis and fluorography. In a beta-lactamase producer, S. cacaoi, at least five membrane-bound penicillin-binding proteins were detected, but in two beta-lactam producers, S. olivaceus and S. clavuligerus, fewer(More)
Productivity and property of beta-lactamases of Streptomyces strains isolated from soil some 30 years ago were studied in comparison with those of the newly isolated strains. At least three-quarters of the Streptomyces strains produced beta-lactamase constitutively and extracellularly, mainly as penicillinases, as in the cases of those from the newly(More)
The methanolic extract of the underground part of Rhodiola sacra (PRAIN ex HAMET) S. H. Fu was found to show inhibitory activity on the histamine release from rat peritoneal exudate cells induced by an antigen-antibody reaction. From the methanolic extract with the inhibitory activity on histamine release, a new cyanoglycoside called rhodiocyanoside D and(More)
On the membrane-bound penicillin-binding proteins (PBP) of Streptomyces cacaoi and S. clavuligerus, the kinetics of [14C]benzylpenicillin binding, the temperature sensitivity, the release of [14C]benzylpenicillin from the [14C]benzylpenicillin-PBP complexes and the changes of the PBP patterns during the growth cycle were examined. All the PBP in both(More)
Penicillin-binding proteins (PBPs) in membrane of Streptomyces cacaoi were investigated by sodium dodecylsulfate-polyacrylamide gel electrophoresis-fluorography. At the same time, eleven beta-lactams were examined on the affinities for these PBPs and the antibacterial activities against S. cacaoi, comparing with those in Bacillus subtilis reported in the(More)
The regulation of expression of the gamma-S-adenosyl-L-methionine (AdoMet) synthetase gene was investigated in T-cells during G0/G1 transition, as well as throughout the G1 phase. Stimulation of G0 T-lymphocytes with concanavalin A induces AdoMet synthetase gene expression, starting 8 h after stimulation. Interleukin-2 (IL-2) stimulates the induction of(More)
A beta-lactamase from culture supernatant of Streptomyces cellulosae was purified about 1,450-fold to apparent homogeneity in polyacrylamide gel electrophoresis and isoelectric focusing on polyacrylamide gel sheet. The methods used were ammonium sulfate precipitation, CM-52 cellulose ion-exchange chromatography and affinity chromatography on Blue Sepharose(More)
Several beta-lactams were investigated on the affinity for the penicillin-binding proteins (PBPs) and the antibacterial activity in Bacillus subtilis. The beta-lactams such as ampicillin, PS-5, methicillin and SCE-963, which had high affinities for PBP-2 showed strong antibacterial activities and the beta-lactams such as cephamycin C, Y-G19Z-GG and(More)