STEPHEN P . REMILLARD

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Centrin, a approximately or equal to 20 kDa calcium-binding protein also known as caltractin, is a component of centrosome-associated algal flagellar roots capable of calcium-mediated contraction, and is also found in the centrosomes of vertebrate cells. Our analysis of a centrin gene from a protist, the amoeboflagellate Naegleria gruberi, reveals conserved(More)
During differentiation of Naegleria from vegetative amoebae to temporary flagellates, the microtubular cytoskeleton, including two basal bodies and flagella, is assembled de novo. Centrin is an integral component of these basal bodies [Levy et al., 1996, Cell Motil. Cytoskeleton 33: 298-323]. In many organisms, centrin appears to be a constitutive protein,(More)
One of two similar genes in the unicellular eukaryote Naegleria gruberi is shown to encode calcineurin B (CnB), the regulatory subunit of calcium-calmodulin-regulated protein phosphatase 2B. Over a span of 156 amino acids, excluding divergent N-termini, the encoded sequence shows 62% identity with vertebrate CnB, and also shows sequence elements specific,(More)
Genes that direct the programmed synthesis of flagellar alpha-tubulin during the differentiation of Naegleria gruberi from amebae to flagellates have been cloned, and found to be novel with respect to gene organization, sequence, and conservation. The flagellar alpha-tubulin gene family is represented in the genome by about eight homologous DNA segments(More)
We labeled gametes of Chlamydomonas with 10-min pulses of 35SO4(-2) before and at various times after deflagellation, and isolated whole cells and flagella immediately after the pulse. The labeled proteins were separated by one- or two-dimensional gel electrophoresis, and the amount of isotope incorporated into specific proteins was determined. Individual(More)
Two calmodulins are synthesized during differentiation of Naegleria gruberi from amoebae to flagellates; one remains in the cell body and the other becomes localized in the flagella. The single, intronless, expressed gene for flagellar calmodulin has been cloned and sequenced. The encoded protein is a typical calmodulin with four putative calcium-binding(More)
A gene that directs the programmed synthesis of flagellar beta-tubulin during the rapid differentiation of Naegleria gruberi from amoebae to flagellates has been cloned and sequenced. The intronless gene is one of 8 to 10 similar but non-identical genes that are dispersed in the genome. beta-Tubulin mRNA homologous to this gene family is expressed(More)
Welabeled gametes of Chlamydomonas with 10-min pulses of S04-' before and at various times after deflagellation, and isolated whole cells and flagella immediately after the pulse . The labeled proteins were separated by oneor two-dimensional gel electrophoresis, and the amount of isotope incorporated into specific proteins was determined . Individual(More)
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