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We demonstrate the presence of cytochrome P4502E1 (CYP2E1) in astrocytes in primary culture, its induction by ethanol, and the concomitant generation of free radical species. Double immunofluorescence using anti-CYP2E1 and anti-glial fibrillary acidic protein showed that CYP2E1 was distributed over the cytoplasm and processes, although labeling was more(More)
Astroglial cells play an important role in maintaining neuronal function in the adult and in the developing nervous system. Ethanol exposure induces profound alterations in the astrogliogenesis process, affecting important cell functions, including intracellular protein trafficking. Because the actin cytoskeleton plays a crucial role in intracellular(More)
The effect of chronic and in vitro ethanol exposure on brain oxygen radical formation and lipid peroxidation was analyzed. Ethanol induces a dose-dependent increase in lipid peroxidation in brain homogenates. The peroxidative effects of alcohol seem to be related to both cytochrome P450 and the ethanol-inducible form of cytochrome P450 (CYP2E1), because(More)
In the present study we analyze the events which occur during the early stages of astrogliogenesis by examining the pattern of both GFAP and vimentin gene expression and their corresponding immunoreactive proteins during rat brain development. This study was carried out "in vivo" (whole brain) and "in vitro" (primary culture of radial glia) using(More)
We have analyzed the effect of prenatal exposure to alcohol on the binding, internalization and secretion of NGF as well as on the content of the NGF receptor (NGFr) in cortical rat astrocytes in primary culture. Secretion of NGF was approximately 1.8-fold greater in 6-day control astrocytes than in 13-day cells. Intracellular content of NGF was very low.(More)
Exposure to ethanol during fetal development reduces the astroglial-specific marker glial fibrillary acidic protein (GFAP) and its mRNA levels in brains of fetal rats and in radial glia in primary culture, affecting the proliferation and differentiation of astrocytes. The objectives of this study were to evaluate the possible effect of ethanol on GFAP mRNA(More)
The alterations in astrocyte proliferation and differentiation induced by prenatal exposure to alcohol (PEA) suggest that ethanol exposure affects the radial glial cells, the main astrocytic precursors. We have investigated the effects of ethanol on the early stages of astrogliogenesis by analyzing the developmental pattern of vimentin and glial fibrillary(More)
Glutamate receptors play important roles during brain development. We have investigated the effect of chronic maternal alcohol intake on the ontogenic profile of hippocampal glutamate receptor subtypes in their offspring. Binding of 3H-MK-801 to N-methyl-D-aspartate (NMDA) receptor was measured in isolated membranes from the hippocampus of the offspring of(More)