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A recent study demonstrated that intracellular small/microRNAs are released from cells, and some of these extracellular RNAs are embedded in vesicles, such as ceramide-rich exosomes, on lipid-bilayer membranes. In the present study, we examined the effects of sphingomyelin phosphodiesterase 3 (SMPD3), which generates ceramide from sphingomyelin, on the(More)
The rat vas deferens was stored overnight in cold, K+-free Krebs solution to deplete intracellular K+ then incubated in K+-, Rb+-, or Cs+-containing Krebs solution at 37 degrees C to load these ions inside the cells. After 4 h, the contents of K+ or Rb+ reached the level of K+ in the fresh vas deferens; the content of Cs+ was less than half that of the(More)
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