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Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance
TLDR
The modified Arabidopsis thaliana miR159 precursor is modified to express artificial miRNAs (amiRNAs) targeting viral mRNA sequences encoding two gene silencing suppressors, P69 of turnip yellow mosaic virus (TYMV) and HC-Pro ofturnip mosaicirus (TuMV).
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Complete nucleotide sequence and genetic organization of papaya ringspot virus RNA.
TLDR
The highly conserved sequence AAAUAAAANANCUCAACACAACAUA at the 5' end of the RNA of PRSV and those of the other five reported potyviruses shows 80% similarity, suggesting that this region may play a common important role for potyvirus replication.
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Nucleotide sequence of the N gene of watermelon silver mottle virus, a proposed new member of the genus Tospovirus
TLDR
The low-degree homology of the N gene with other tospoviruses coupled with the lack of cross hybridization using a Tospo-W-specific cDNA probe suggests that Tospi-W, renamed as watermelon silver mottle virus, is a distinct member of the genus Tospovirus.
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Control of papaya ringspot virus by cross protection
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Efficient rooting for establishment of papaya plantlets by micropropagation
TLDR
Root induction in low-concentration IBA agar medium followed by root development in vermiculite containing half strength MS medium under aerated conditions results in efficient rooting of in vitro papaya shoots.
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Broad-Spectrum Resistance to Different Geographic Strains of Papaya ringspot virus in Coat Protein Gene Transgenic Papaya.
TLDR
Molecular analysis of nine selected transgenic papaya lines with broad-spectrum resistance revealed that the expression level of the transgene is negatively correlated with the degree of resistance, suggesting that the resistance is manifested by a RNA-mediated mechanism.
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A Chlorotic Spot Disease on Calla Lilies (Zantedeschia spp.) Is Caused by a Tospovirus Serologically but Distantly Related to Watermelon silver mottle virus.
TLDR
Results of RT-PCR show that the conserved regions of the L genes of tospoviruses are present in CCSV, and the virus was weakly related to Watermelon silver mottle virus (WSMoV) in enzyme-linked immunosorbent assay (ELISA) and western blot tests.
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Identification of tomato spotted wilt-like virus on watermelon in Taiwan
The causal agent of an unusual disease affecting watermelon (Citrullus lanatus) in Taiwan was identified as tomato spotted wilt-like virus in the tospovirus group on the basis of particle morphology,…
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Comparative reactions of recombinant papaya ringspot viruses with chimeric coat protein (CP) genes and wild-type viruses on CP-transgenic papaya.
TLDR
It is concluded that the position of the heterologous sequences in the recombinants influences their pathogenicity on Rainbow, and the role of CP sequence similarity in overcoming the resistance of Rainbow is investigated.
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Comparison of the Nuclear Inclusion b Protein and Coat Protein Genes of Five Papaya Ringspot Virus Strains Distinct in Geographic Origin and Pathogenicity
A complementary DNA library was constructed to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb…
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