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Unique palindromic sequences in synthetic oligonucleotides are required to induce IFN [correction of INF] and augment IFN-mediated [correction of INF] natural killer activity.
- S. Yamamoto, T. Yamamoto, T. Kataoka, E. Kuramoto, O. Yano, T. Tokunaga
- Biology, ChemistryJournal of immunology
- 15 June 1992
The results strongly suggest that the presence of the unique palindromic sequences, such as GACGTC, AGCGCT, and AACGTT, but not ACCGGT, is essential for the immunostimulatory activity of oligonucleotides.
Oligodeoxynucleotides containing palindrome sequences with internal 5'-CpG-3' act directly on human NK and activated T cells to induce IFN-gamma production in vitro.
The results indicate that oligo-DNAs containing CG palindrome act directly on human NK cells and activated T cells to induce IFN-gamma production.
Binding of oligoguanylate to scavenger receptors is required for oligonucleotides to augment NK cell activity and induce IFN.
It is suggested that the binding of an extrapalindromic sequence to the scavenger receptor is required for the immunostimulatory activity of oligo-1, a 30mer single-stranded oligonucleotide with oligoG sequences next to the active palindromo-G sequences.
Analysis of the Role of TFIIE in Transcriptional Regulation through Structure-Function Studies of the TFIIEβ Subunit*
It is demonstrated that two C-terminal basic regions are essential for physical interaction with both TFIIEα and single-stranded DNA, as well as with other transcription factors including the Drosophila transcriptional regulator Krüppel.
Visualization of mitochondria with green fluorescent protein in cultured fibroblasts from patients with mitochondrial diseases.
- M. Kanazawa, M. Yano, H. Niimi
- Biology, MedicineBiochemical and biophysical research…
- 20 October 1997
Transfected cDNAs for green fluorescent protein and a GFP fusion protein containing the presequence of human ornithine transcarbamylase were transfected into cultured human fibroblasts and gave mitochondria-associated fluorescence, useful to analyze mitochondrial structural alterations and disorders of mitochondrial protein import.
Ability of oligonucleotides with certain palindromes to induce interferon production and augment natural killer cell activity is associated with their base length.
It is indicated that the immunostimulatory activity of oligonucleotides with certain palindromic sequences requires an oligon nucleotide at least 18 bases long.
High-level and effective production of human mannan-binding lectin (MBL) in Chinese hamster ovary (CHO) cells.
Hexamer palindromic oligonucleotides with 5'-CG-3' motif(s) induce production of interferon.
- K. Sonehara, H. Saito, E. Kuramoto, S. Yamamoto, T. Yamamoto, T. Tokunaga
- Biology, ChemistryJournal of interferon & cytokine research : the…
- 1 October 1996
The results strongly suggest that the minimal essential structure required for IFN induction is the hexamer palindromic sequence with CG motif(s), and that oligonucleotides with NACGTN and NTCGAN sequences exhibited the strongest activity.
Site-directed mutagenesis of recombinant rat DNA polymerase beta: involvement of arginine-183 in primer recognition.
- T. Date, S. Yamamoto, K. Tanihara, Y. Nishimoto, N. Liu, A. Matsukage
- Biology, ChemistryBiochemistry
- 29 May 1990
It is concluded that Arg-183 occupies an important part of the primer recognition site of DNA polymerase beta.
Oligodeoxyribonucleotides with 5'-ACGT-3' or 5'-TCGA-3' sequence induce production of interferons.
- S. Yamamoto, T. Yamamoto, T. Tokunaga
- Biology, MedicineCurrent topics in microbiology and immunology
Evidence foreshadowing the finding of senseless immunostimulatory DNA was also obtained by the experimental use of BCG.