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Diagnosis of plant viral pathogens
Current diagnosis techniques are broadly divided into serological procedures, nucleic acid procedures and combinations of both, and upcoming methods are presented.
A polymerase chain reaction assay for cucumber mosaic virus in lupin seeds
A Polymerase Chain Reaction using ground dry seed samples was developed, which detected CMV in 16 commercial seed samples belonging to seven cultivars from 12 different localities and was suitable for routine testing of the flour derived from grinding dry seed.
Zucchini yellow mosaic virus: biological properties, detection procedures and comparison of coat protein gene sequences
- B. Coutts, M. Kehoe, C. Webster, S. Wylie, R. Jones
- Biology, MedicineArchives of Virology
- 21 September 2011
Findings suggest that there have been at least three separate ZYMV introductions into Australia and there are few changes to local isolate CP sequences following their establishment in remote growing areas.
ICTV Virus Taxonomy Profile: Potyviridae
- S. Wylie, M. Adams, +8 authors F. Zerbini
- Biology, MedicineThe Journal of general virology
- 1 March 2017
The Potyviridae is the largest family of RNA plant viruses, members of which have single-stranded, positive-sense RNA genomes and flexuous filamentous particles 680–900 nm long and 11–20 nm wide.…
Phylogenetic Analysis of Bean yellow mosaic virus Isolates from Four Continents: Relationship Between the Seven Groups Found and Their Hosts and Origins.
A plausible explanation for the groups found when the 64 different CP sequences were compared is that the generalized group represents the original ancestral type from which the specialist host groups evolved in response to domestication of plants after the advent of agriculture.
Virus impact at the interface of an ancient ecosystem and a recent agroecosystem: studies on three legume‐infecting potyviruses in the southwest Australian floristic region
How introduced viruses can damage indigenous plants and indigenous virus can damage introduced cultivated plants within this unique ecosystem is illustrated, and how human activities can facilitate damaging 'new encounters' between plants and viruses is illustrated.
Transgenic yellow lupin (Lupinus luteus)
The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples
RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology to identify viruses that co-infected the same plant.
Complete genome sequences of seven carlavirus and potyvirus isolates from Narcissus and Hippeastrum plants in Australia, and proposals to clarify their naming
Proposals are made to clarify the current confusion surrounding the naming of some of the potyviruses narcissus degeneration virus, narcissus yellow stripe virus and Vallota speciosa virus from narcissus plants.
Localized Distribution of Iris yellow spot virus Within Leeks and Its Reliable Large-Scale Detection.
In a survey to determine the incidence of Iris yellow spot virus (IYSV) in crops of several host species, samples of one leaf tip/plant were collected at random and tested by enzyme-linked immunosorbent assay (ELISA), the virus was detected only in leek and onion.