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Molecular views of recombination proteins and their control
  • S. West
  • Biology, Medicine
  • Nature Reviews Molecular Cell Biology
  • 1 June 2003
The efficient repair of double-strand breaks in DNA is critical for the maintenance of genome stability and cell survival, especially in replicating cells, in which it plays a major role in tumour avoidance. Expand
Role of BRCA2 in control of the RAD51 recombination and DNA repair protein.
It is shown that BRCA2 plays a dual role in regulating the actions of RAD51, a protein essential for homologous recombination and DNA repair, which may be a key event leading to genomic instability and tumorigenesis in patients predisposed to breast and ovarian cancers. Expand
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
It is suggested that the extreme sensitivity of paralog-defective cell lines to cross-linking agents is owing to defects in the processing of incised cross links and the consequential failure to initiate recombinational repair at these sites. Expand
Identification of Holliday junction resolvases from humans and yeast
The human Holliday junction resolvase, GEN1, and its yeast orthologue, Yen1, were independently identified using two distinct experimental approaches: GEN1 was identified by mass spectrometry following extensive fractionation of HeLa cell-free extracts, whereas Yen1 was detected by screening a yeast gene fusion library for nucleases capable of Holliday junctions resolution. Expand
DNA interstrand crosslink repair and cancer
Understanding how the FA pathway links nucleases, helicases and other DNA-processing enzymes should lead to more targeted uses of ICL-inducing agents in cancer treatment and could provide novel insights into drug resistance. Expand
Regulatory Control of the Resolution of DNA Recombination Intermediates during Meiosis and Mitosis
It is shown that the specialized chromosome segregation patterns of meiosis and mitosis are achieved by regulating the timing of activation of two crossover-promoting endonucleases, which facilitates chromosome segregation while limiting the potential for loss of heterozygosity and sister-chromatid exchanges. Expand
Poly(ADP-ribose)–Dependent Regulation of DNA Repair by the Chromatin Remodeling Enzyme ALC1
A chromatin-remodeling enzyme, ALC1 (Amplified in Liver Cancer 1, also known as CHD1L), is identified that interacts with poly(ADP-ribose) and catalyzes PARP1-stimulated nucleosome sliding and provides new insights into the mechanisms by which poly(adenosine 5′-diphosphate (ADP)–riboses) regulates DNA repair. Expand
Structure of the single-strand annealing domain of human RAD52 protein
The crystal structure of the N-terminal fragment of human RAD52 reveals an undecameric subunit ring with extensive subunit contacts, readily suggesting a mechanism by which RAD52 presents the single strand for reannealing with complementary single-stranded DNA. Expand
Human Rad51 Protein Promotes ATP-Dependent Homologous Pairing and Strand Transfer Reactions In Vitro
The results establish hRad51 as a functional homolog of RecA, but indicate that the human protein and its bacterial counterpart differ in their ability to promote extensive strand transfer. Expand
CDK-dependent phosphorylation of BRCA2 as a regulatory mechanism for recombinational repair
It is shown that the carboxy-terminal region of BRCA2, which interacts directly with the essential recombination protein RAD51, contains a site that is phosphorylated by cyclin-dependent kinases, indicating that S3291 phosphorylation might provide a molecular switch to regulate RAD51 recombination activity. Expand