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Snake Envenoming: A Disease of Poverty
- R. Harrison, A. Hargreaves, S. Wagstaff, B. Faragher, D. Lalloo
- Medicine, EconomicsPLoS neglected tropical diseases
- 1 December 2009
This study unequivocally demonstrates that snake envenoming is a disease of the poor, and the negative association between snakebite deaths and government expenditure on health confirms that the burden of mortality is highest in those countries least able to deal with the considerable financial cost of snakebite.
Comparative venom gland transcriptome surveys of the saw-scaled vipers (Viperidae: Echis) reveal substantial intra-family gene diversity and novel venom transcripts
Comparison of Echis venom gland transcriptomes revealed substantial intrageneric venom variation in representations and cluster numbers of the most abundant venom toxin families, suggesting alterations in isoform diversity or transcript expression levels within the major venom protein families are likely to be responsible for prey specificity.
Medically important differences in snake venom composition are dictated by distinct postgenomic mechanisms
- N. Casewell, S. Wagstaff, R. Harrison
- BiologyProceedings of the National Academy of Sciences
- 9 June 2014
Assessment of the venom composition of six related viperid snakes reveals that multiple levels of regulation are responsible for generating variation in venom composition between related snake species, and demonstrates how this variability can undermine the treatment of a neglected tropical disease, snakebite.
Bioinformatics and Multiepitope DNA Immunization to Design Rational Snake Antivenom
A novel bioinformatic strategy that identified sequences encoding immunogenic and structurally significant epitopes from an expressed sequence tag database of a venom gland cDNA library of Echis ocellatus, the most medically important viper in Africa, is developed and adapted to immunotherapy design in other cases where targets are numerous, diverse, and poorly characterized such as those generated by hypermutation or antigenic variation.
Domain loss facilitates accelerated evolution and neofunctionalization of duplicate snake venom metalloproteinase toxin genes.
- N. Casewell, S. Wagstaff, R. Harrison, Camila Renjifo, W. Wüster
- BiologyMolecular biology and evolution
- 1 September 2011
This work investigates the role alterations in domain structure have on the tempo of evolution and neofunctionalization of multigene families using the snake venom metalloproteinases (SVMPs) as a model system and details how the loss of domain structures can catalyze the accelerated evolution of novel gene paralogues.
Unusual Stability of Messenger RNA in Snake Venom Reveals Gene Expression Dynamics of Venom Replenishment
- Rachel B Currier, J. Calvete, L. Sanz, R. Harrison, P. Rowley, S. Wagstaff
- BiologyPloS one
- 7 August 2012
It is demonstrated that venom re-synthesis occurs very rapidly following depletion of venom stores, presumably to ensure venomous snakes retain their ability to efficiently predate and remain defended from predators.
Extracellular ATP activates multiple signalling pathways and potentiates growth factor-induced c-fos gene expression in MCF-7 breast cancer cells.
Extracellular nucleotides co-operate with growth factors to activate genes linked to the proliferative response in MCF-7 cells through activation of specific purinergic receptors, which thereby represent important potential targets for arresting the neoplastic progression of breast cancer cells.
Parathyroid Hormone Potentiates Nucleotide-induced [Ca2+] i Release in Rat Osteoblasts Independently of Gq Activation or Cyclic Monophosphate Accumulation
- K. A. Buckley, S. Wagstaff, W. B. Bowler
- Biology, ChemistryThe Journal of Biological Chemistry
- 23 March 2001
It is reported that the localized release of nucleotides can sensitize osteoblasts to the activity of systemic factors, and have important implications for PTH-induced signaling in bone.
Neurotrophin‐4 delivered by fibrin glue promotes peripheral nerve regeneration
Morphometric analysis revealed that nerves treated with NT‐4 had significant improvement in the number of regenerated axons, axonal diameter, and myelin thickness, suggesting thatNT‐4 is a potent factor improving rat sciatic nerve regeneration.