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Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda)
DNA barcoding is proposed as a reliable, consistent, and democratic tool for species discrimination in routine identification of parasitic nematodes and the coherence between DNA-based and morphological identification for almost all the species examined is very strong. Expand
Localization of Plasmodium falciparum histidine-rich protein 1 in the erythrocyte skeleton under knobs.
Immunofluorescence and immunoelectron microscopy of intact, non-fixed K+ infected erythrocytes using mAb 89 and a rabbit antiserum raised against purified PfHRP-1, failed to identify any surface exposed epitopes. Expand
New Insights into the Evolution of Wolbachia Infections in Filarial Nematodes Inferred from a Large Range of Screened Species
The absence of Wolbachia in 63% of onchocercids, notably in the ancestral Oswaldofilariinae estimated 140 mya old, the diverse tissues or specimens distribution, and a recent lateral transfer in supergroup F WolbachIA modify the current view on the role and evolution of the endosymbiont and their hosts. Expand
Secretion of a malarial histidine-rich protein (Pf HRP II) from Plasmodium falciparum-infected erythrocytes
Results provide evidence for an intracellular route of transport for a secreted malarial protein from the parasite through several membranes and the host cell cytoplasm. Expand
Isolation of a Plasmodium falciparum rhoptry protein.
A monoclonal antibody raised against the malaria parasite Plasmodium falciparum recognised a protein of 140000 molecular weight which was synthesized during schizogony, which is located in the rhoptries, the apical organelles of the merozoite. Expand
Immune responses to Cryptosporidium muris and Cryptosporidium parvum in adult immunocompetent or immunocompromised (nude and SCID) mice
The results suggested that the two animal models should be valuable in the study of immunity to cryptosporidial infection. Expand
Both asymmetric mitotic segregation and cell-to-cell invasion are required for stable germline transmission of Wolbachia in filarial nematodes
Multiple evolutionarily conserved mechanisms that are required for Wolbachia localization to the germline are revealed, including the utilization of cell-to-cell invasion for germline colonization and an ancestral mode of horizontal transfer that preceded the acquisition of the mutualism. Expand
Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan.
Cystoisospora spp. from feces in dogs, cats, and raccoon dogs were isolated, sequenced at the small subunit ribosomal RNA gene locus and compared to other Cystoisospora spp. Cystoisospora oocystsExpand
A new type F Wolbachia from Splendidofilariinae (Onchocercidae) supports the recent emergence of this supergroup.
The phylogenetic analyses confirmed a recent emergence of supergroup F Wolbachia and suggested several events of horizontal transmission between nematodes and arthropods in this supergroup, and the existence of different metabolic interactions between the filarial nem atodes and their symbionts. Expand
Transport of an Mr approximately 300,000 Plasmodium falciparum protein (Pf EMP 2) from the intraerythrocytic asexual parasite to the cytoplasmic face of the host cell membrane
The results show that the intraeriesthrocytic malaria parasite has evolved a novel system for transporting malarial proteins beyond its own plasma membrane, through a vacuolar membrane and the host erythrocyte cytoplasm to the ery throat membrane, where they become membrane bound and presumably alter the properties of this membrane to the parasite's advantage. Expand