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TYROSINE HYDROXYLASE. THE INITIAL STEP IN NOREPINEPHRINE BIOSYNTHESIS.
TLDR
It has now been possible to demonstrate that brain, adrenal medulla, and sympathetically innervated tissues contain a specific hydroxylase that catalyzes the conversion of L-tyrosine to dopa. Expand
ELUCIDATION OF THE RATE-LIMITING STEP IN NOREPINEPHRINE BIOSYNTHESIS IN THE PERFUSED GUINEA-PIG HEART.
TLDR
Factors indicate that conversion of tyrosine to dopa is the rate-limiting step in the formation of norepinephrine in the sympathetic nervous system and ascorbic acid has been shown to be a requirement for purified dopamine-β-oxidase activity. Expand
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products areExpand
How glycosylphosphatidylinositol-anchored membrane proteins are made.
TLDR
Studying in whole cells and in cell-free systems indicate that structural requirements around the COOH-terminal cleavage site of nascent proteins are similar to those at the cleavage sites of NH2- terminal signal peptidase, however, COOh-terminAL processing requires a transmidase for which evidence is presented as well as a proposed mechanism of its action. Expand
A fluorometric method for the estimation of tyrosine in plasma and tissues.
TLDR
The fluorometric method is used to determine the amount of tyrosine in plasma from fasting patients with different disease states, as compared to the amountof the amino acid found in the plasma of normal fasting controls. Expand
Aromatic L-amino acid decarboxylase.
The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase.
TLDR
Reconstitution of class K cells with hGPI8 abolishes their accumulation of G PI precursors and restores C-terminal processing of GPI-anchored proteins, and restores the ability of microsomes from the mutant cells to yield an active carbonyl in the presence of a proprotein, considered to be an intermediate in catalysis by a transamidase. Expand
PRINCIPLES OF FLUORESCENCE
This chapter discusses the principles of fluorescence. Fluorescence is only one manifestation of the interaction of electromagnetic radiation with matter and that along with phosphorescence it canExpand
Fluorometric assay of proteins in the nanogram range.
TLDR
The reagent fluorescamine has been used for the fluorometric assay of proteins based on their content of free amino groups and its applications to the monitoring of proteins during enzyme purification are described. Expand
The use of dopamine -hydroxylase as a marker for the central noradrenergic nervous system in rat brain.
TLDR
Improvements in the method for localization of dopamine-beta-hydroxylase by immunofluorescence allow the observation of noradrenergic-cell bodies, non-terminal fibers, and axon terminals in the rat brain, and these observations suggest that cerebral microcirculation is regulated by central nor adrenergic neurons. Expand
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