TYROSINE HYDROXYLASE. THE INITIAL STEP IN NOREPINEPHRINE BIOSYNTHESIS.
- T. Nagatsu, M. Levitt, S. Udenfriend
- Biology, ChemistryJournal of Biological Chemistry
- 1 September 1964
It has now been possible to demonstrate that brain, adrenal medulla, and sympathetically innervated tissues contain a specific hydroxylase that catalyzes the conversion of L-tyrosine to dopa.
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
- S. Udenfriend, S. Stein, Peter B�hlen, W. Dairman, W. Leimgruber, M. Weigele
- Chemistry, BiologyScience
- 24 November 1972
Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are…
ELUCIDATION OF THE RATE-LIMITING STEP IN NOREPINEPHRINE BIOSYNTHESIS IN THE PERFUSED GUINEA-PIG HEART.
- M. Levitt, S. Spector, A. Sjoerdsma, S. Udenfriend
- Biology, ChemistryJournal of Pharmacology and Experimental…
- 1 April 1965
Factors indicate that conversion of tyrosine to dopa is the rate-limiting step in the formation of norepinephrine in the sympathetic nervous system and ascorbic acid has been shown to be a requirement for purified dopamine-β-oxidase activity.
How glycosylphosphatidylinositol-anchored membrane proteins are made.
Studying in whole cells and in cell-free systems indicate that structural requirements around the COOH-terminal cleavage site of nascent proteins are similar to those at the cleavage sites of NH2- terminal signal peptidase, however, COOh-terminAL processing requires a transmidase for which evidence is presented as well as a proposed mechanism of its action.
A fluorometric method for the estimation of tyrosine in plasma and tissues.
The fluorometric method is used to determine the amount of tyrosine in plasma from fasting patients with different disease states, as compared to the amountof the amino acid found in the plasma of normal fasting controls.
Aromatic L-amino acid decarboxylase.
Fluorometric assay of proteins in the nanogram range.
PRINCIPLES OF FLUORESCENCE
- S. Udenfriend
- Chemistry, Biology
The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase.
- J. Yu, S. Nagarajan, J. Knez, S. Udenfriend, R. Chen, M. Medof
- BiologyProceedings of the National Academy of Sciences…
- 11 November 1997
Reconstitution of class K cells with hGPI8 abolishes their accumulation of G PI precursors and restores C-terminal processing of GPI-anchored proteins, and restores the ability of microsomes from the mutant cells to yield an active carbonyl in the presence of a proprotein, considered to be an intermediate in catalysis by a transamidase.