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Trapping of PARP1 and PARP2 by Clinical PARP Inhibitors.
TLDR
This study shows that PARP inhibitors trap the PARP1 and PARP2 enzymes at damaged DNA, providing a new mechanistic foundation for the rational application ofPARP inhibitors in cancer therapy. Expand
Homologous recombination and non‐homologous end‐joining pathways of DNA double‐strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells
TLDR
Observations provide the first genetic evidence that both repair pathways play a role in maintaining chromosomal DNA during the cell cycle. Expand
Chromosome Instability and Defective Recombinational Repair in Knockout Mutants of the Five Rad51 Paralogs
TLDR
It is concluded that the Rad51 paralogs participate in repair as a functional unit that facilitates the action of Rad51 in HR and shows partial correction of resistance to DNA damage by overexpression of human Rad51. Expand
DNA Damage-Dependent Acetylation and Ubiquitination of H2AX Enhances Chromatin Dynamics
TLDR
It is shown that ionizing irradiation induces TIP60 acetylation of histone H2AX, a variant form of H2A known to be phosphorylated following DNA damage, and ubiquitination via the ubiquitin-conjugating enzyme UBC13, which is induced by DNA damage. Expand
Rad51‐deficient vertebrate cells accumulate chromosomal breaks prior to cell death
TLDR
Chromosome analysis revealed that most metaphase‐arrested Rad51− cells carried isochromatid‐type breaks, indicating that Rad51 fulfils an essential role in the repair of spontaneously occurring chromosome breaks in proliferating cells of higher eukaryotes. Expand
A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination.
TLDR
It is shown that disruption or siRNA depletion of UBC13 in chicken DT40 or human cells confers severe growth defects due to chromosome instability, and hypersensitivity to both UV and ionizing radiation, consistent with a conserved role for Ubc13 in PRR. Expand
Stereospecific PARP Trapping by BMN 673 and Comparison with Olaparib and Rucaparib
TLDR
BMN 673 is the most potent clinical PARP inhibitor tested to date with the highest efficiency at trapping PARP–DNA complexes and is also approximately 100-fold more cytotoxic than olaparib and rucaparIB in combination with the DNA alkylating agents methyl methane sulfonate and temozolomide. Expand
Cyclin-dependent kinases and cell-cycle transitions: does one fit all?
TLDR
This work discusses cell-cycle control in light of overlapping and essential functions of the different CDKs and cyclin subunits in higher eukaryotes. Expand
REV1 protein interacts with PCNA: significance of the REV1 BRCT domain in vitro and in vivo.
TLDR
In vivo studies in both chicken DT40 cells and yeast directly support the requirement of the BRCT domain of REV1 for cell survival and DNA damage-induced mutagenesis. Expand
Inhibition of homologous recombination by the PCNA-interacting protein PARI.
TLDR
It is reported that the protein PARI, containing a UvrD-like helicase domain, is a PCNA-interacting partner required for preservation of genome stability in human and DT40 chicken cells and suggested to be a long sought-after factor that suppresses inappropriate recombination events at mammalian replication forks. Expand
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