• Publications
  • Influence
Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B
The structures of BoNT/B and its complex with sialyllactose provide a detailed description of the active site and a model for interactions between the toxin and its cell surface receptor, and the latter may provide valuable information for recombinant vaccine development. Expand
Crystal structure of staphylococcal enterotoxin B, a superantigen
The three-dimensional structure of staphylococcal enterotoxin B, which is both a toxin and a super-antigen, has been determined to a resolution of 2.5 Å. The unusual main-chain fold containing twoExpand
Domain organization in Clostridium botulinum neurotoxin type E is unique: its implication in faster translocation.
It is suggested that the translocation domain in other BoNTs follows a two-step process to attain translocation-competent conformation as in BoNT E, and this is a probable reason for its faster toxic rate compared to BoNT A. Expand
Mechanism of action of a flavin-containing monooxygenase.
It is proposed that FMOs exist in the cell as a complex with a reduced form of the prosthetic group and NADPH cofactor, readying them to act on substrates to eliminate nonnutritional and insoluble compounds. Expand
Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi
The surface of OspC that would project away from the spirochete's membrane has a region of strong negative electrostatic potential which may be involved in binding to positively charged host ligands. Expand
Crystal structure of a putative CN hydrolase from yeast
Although the precise enzymatic reactions catalyzed by the yeast protein are not known, considerable information about the active site may be deduced from conserved sequence motifs, comparative biochemical information, and comparison with known structures of hydrolase active sites. Expand
Crystal structure of trehalose‐6‐phosphate phosphatase–related protein: Biochemical and biological implications
We report here the crystal structure of a trehalose‐6‐phosphate phosphatase–related protein (T6PP) from Thermoplasma acidophilum, TA1209, determined by the dual‐wavelength anomalous diffraction (DAD)Expand
Substrate Binding Mode and Its Implication on Drug Design for Botulinum Neurotoxin A
The crystal structures of the catalytic domain of BoNT/A with its uncleavable SNAP-25 peptide and its variant 197RRATKM202 to 1.5 Å and 1.6 Å are reported, which should form the basis for design of potent inhibitors for this neurotoxin. Expand
Structural genomics: beyond the Human Genome Project
A program of high-throughput X-ray crystallography aimed at developing a comprehensive mechanistic understanding of normal and abnormal human and microbial physiology at the molecular level is about to embark on. Expand
Structure of the receptor binding fragment HC of tetanus neurotoxin
The 2.7 A structure of the tetanus neurotoxin receptor binding fragment Hc reveals a jelly-roll domain and a beta-trefoil domain. Hc retains the unique transport properties of the holotoxin and isExpand