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Conserved noncoding genomic sequences associated with a flowering-time quantitative trait locus in maize
Flowering time is a fundamental trait of maize adaptation to different agricultural environments. Although a large body of information is available on the map position of quantitative trait loci forExpand
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Complete sequence analysis of transgene loci from plants transformed via microprojectile bombardment
A substantial literature exists characterizing transgene locus structure from plants transformed via Agrobacterium and direct DNA delivery. However, there is little comprehensive sequence analysis ofExpand
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Artificial chromosome formation in maize (Zea mays L.)
We report on the construction of maize minichromosomes using shuttle vectors harboring native centromeric segments, origins of replication, selectable marker genes, and telomeric repeats. TheseExpand
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Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus.
We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St, Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences,Expand
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Complex transgene locus structures implicate multiple mechanisms for plant transgene rearrangement.
To more fully characterize the internal structure of transgene loci and to gain further understanding of mechanisms of transgene locus formation, we sequenced more than 160 kb of complex transgeneExpand
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Genomic interspersions determine the size and complexity of transgene loci in transgenic plants produced by microprojectile bombardment.
The structure of transgene loci in six transgenic allohexaploid oat (Avena sativa L.) lines produced using microprojectile bombardment was characterized using fluorescence in situ hybridizationExpand
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Association of transgene integration sites with chromosome rearrangements in hexaploid oat
Abstract Transgene loci in 16 transgenic oat (Avena sativa L.) lines produced by microprojectile bombardment were characterized using phenotypic and genotypic segregation, Southern blot analysis, andExpand
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A study of 28 Elymus species using repetitive DNA sequences.
Four repetitive DNA sequences cloned from the barley (Hordeum vulgare) genome and common for different Triticeae species were used for a molecular study of phylogenetic relationships among 28 ElymusExpand
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Microsatellite megatracts in the maize (Zea mays L.) genome.
Long tracts (megatracts) of (CAG)n, (TAG)n, and (GAA)n microsatellite sequences capable of forming composite DNA segments were found in the maize (Zea mays L.) genome. Some of the (CAG)n and (TAG)nExpand
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Characterization of transgene loci in plants using FISH: A picture is worth a thousand words
Transgenic plants are traditionally characterized using phenotypic and Southern analyses. Over the last several years, fluorescence in situ hybridization (FISH) has been efficiently used forExpand
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