Sequence diversity in CYP3A promoters and characterization of the genetic basis of polymorphic CYP3A5 expression
- P. Kuehl, Jiong Zhang, E. Schuetz
- BiologyNature Genetics
- 1 April 2001
CYP3A5 was more frequently expressed in livers of African Americans than in those of Caucasians, and may be the most important genetic contributor to interindividual and interracial differences in CYP3A-dependent drug clearance and in responses to many medicines.
Stem Cell Characteristics of Amniotic Epithelial Cells
- T. Miki, T. Lehmann, H. Cai, D. Stolz, S. Strom
- Biology, MedicineStem Cells
- 1 November 2005
Amniotic epithelial cells isolated from human term placenta express surface markers normally present on embryonic stem and germ cells, and have the potential to differentiate to all three germ layers—endoderm (liver, pancreas, mesoderm) in vitro and ectoderm in vitro.
Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver
- Meritxell Huch, H. Gehart, H. Clevers
- Biology, MedicineCell
- 15 January 2015
Mapping the Genetic Architecture of Gene Expression in Human Liver
This genome-wide association study of gene expression resulted in the detection of more than 6,000 associations between SNP genotypes and liver gene expression traits, where many of the corresponding genes identified have already been implicated in a number of human diseases.
Robust expansion of human hepatocytes in Fah−/−/Rag2−/−/Il2rg−/− mice
This system provides a robust platform to produce high-quality human hepatocytes for tissue culture and may be useful for testing the toxicity of drug metabolites and for evaluating pathogens dependent on human liver cells for replication.
Hepatic CYP2B6 Expression: Gender and Ethnic Differences and Relationship to CYP2B6 Genotype and CAR (Constitutive Androstane Receptor) Expression
- V. Lamba, J. Lamba, E. Schuetz
- BiologyJournal of Pharmacology and Experimental…
- 1 December 2003
The 1459C>T SNP, which resulted in the Arg487Cys substitution, was associated with the lowest level of CYP2B6 activity in livers of females, and several common SNPs that are associated with polymorphic CYP 2B6 expression were found.
Concise Review: Isolation and Characterization of Cells from Human Term Placenta: Outcome of the First International Workshop on Placenta Derived Stem Cells
- O. Parolini, F. Alviano, S. Strom
- Biology, MedicineStem Cells
- 1 February 2008
The aim of this review is to summarize and provide the state of the art of research in this field, addressing aspects such as cell isolation protocols and characteristics of these cells, as well as providing preliminary indications of the possibilities for use ofThese cells in future clinical applications.
Interactions between Hepatic Mrp4 and Sult2a as Revealed by the Constitutive Androstane Receptor and Mrp4 Knockout Mice*
- M. Assem, E. Schuetz, J. Schuetz
- BiologyJournal of Biological Chemistry
- 21 May 2004
It is suggested that Mrp4 and Sult2a1 participate in an integrated pathway mediating elimination of sulfated steroid and bile acid metabolites from the liver in order to protect the liver from accumulation of hydrophobic bile acids.
The human pregnane X receptor: genomic structure and identification and functional characterization of natural allelic variants.
- J. Zhang, P. Kuehl, M. Boguski
- Biology, MedicinePharmacogenetics (London)
- 1 October 2001
The relevance of each of the 38 PXR SNPs identified in DNA of individuals whose CYP3A basal and rifampin-inducible CYP 3A4 expression was determined in vivo and/or in vitro was demonstrated by univariate statistical analysis.
Disrupted Bile Acid Homeostasis Reveals an Unexpected Interaction among Nuclear Hormone Receptors, Transporters, and Cytochrome P450*
- E. Schuetz, S. Strom, J. Schuetz
- Biology, MedicineJournal of Biological Chemistry
- 19 October 2001
Enhanced hepatocellular concentrations of bile acids, due to the down-regulation of BSEP/SPGP-mediated efflux in FXR nullizygous mice, result in an alternate but apparent compensatory up- regulation of CYP3A, CYP2B, and some ABC transporters that is consistent with activation of PXR/SXR by bile acid.