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A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes
TLDR
The Huntington's disease mutation involves an unstable DNA segment, similar to those described in fragile X syndrome, spino-bulbar muscular atrophy, and myotonic dystrophy, acting in the context of a novel 4p16.3 gene to produce a dominant phenotype. Expand
An induced-fit mechanism to promote peptide bond formation and exclude hydrolysis of peptidyl-tRNA
TLDR
Three new structures of the large ribosomal subunit of Haloarcula marismortui (Hma) complexed with peptidyl transferase substrate analogues are described that reveal an induced-fit mechanism in which substrates and active-site residues reposition to allow the peptidol transferase reaction. Expand
Crystal structure of a self-splicing group I intron with both exons
TLDR
The X-ray crystal structure of a complete group I bacterial intron in complex with both the 5′- and the 3′-exons is reported, representing the first splicing complex to include a complete intron, both exons and an organized active site occupied with metal ions. Expand
Structural insights into the roles of water and the 2' hydroxyl of the P site tRNA in the peptidyl transferase reaction.
TLDR
Crystal structures of the large ribosomal subunit of Haloarcula marismortui (Hma) complexed with several analogs that represent either the substrates or the transition state intermediate of the peptidyl transferase reaction show that this reaction proceeds through a tetrahedral intermediate with S chirality. Expand
Structural investigation of the GlmS ribozyme bound to Its catalytic cofactor.
TLDR
Comparison of this activator bound and the previously published apoenzyme complex supports a model in which GlcN6P does not induce a conformational change in the RNA, as is typical of other riboswitches, but instead functions as a catalytic cofactor for the reaction. Expand
A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits
TLDR
A version of this assay that does not require alcohol is described and used to show, both crystallographically and biochemically, that crystals of the large ribosomal subunits from Haloarcula marismortui are enzymatically active. Expand
Biodegradation of Polyester Polyurethane by Endophytic Fungi
TLDR
The broad distribution of activity observed and the unprecedented case of anaerobic growth using PUR as the sole carbon source suggest that endophytes are a promising source of biodiversity from which to screen for metabolic properties useful for bioremediation. Expand
Important contribution to catalysis of peptide bond formation by a single ionizing group within the ribosome.
TLDR
Results suggest a contribution to overall catalysis of general acid-base and/or conformational catalysis involving an ionizing group at the active site in peptidyl transferase reaction with puromycin. Expand
Structural basis of ligand binding by a c-di-GMP riboswitch
TLDR
Kinetic and structural data suggest that genetic regulation by the c-di-GMP riboswitch is kinetically controlled and that gene expression is modulated through the stabilization of a previously unidentified P1 helix, illustrating a direct mechanism for c- Di GMP signaling. Expand
Substrate-assisted catalysis of peptide bond formation by the ribosome
TLDR
Evidence that a functional group on one of the tRNA substrates plays an essential catalytic role in the reaction is reported, suggesting that substrate assistance has been retained as a catalytic strategy during the evolution of the prebiotic peptidyl transferase center into the modern ribosome. Expand
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