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alpha- and beta-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure.
Protein phosphatase 2A (polycation-stimulated protein phosphatase L) was purified from porcine kidney and skeletal muscle. The 36-kDa catalytic and the 65-kDa putative regulatory (hereafter termed… Expand
The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment.
- W. Bode, I. Mayr, U. Baumann, R. Huber, S. Stone, J. Hofsteenge
- Biology, Medicine
- The EMBO journal
A stoichiometric complex formed between human alpha-thrombin and D-Phe-Pro-Arg chloromethylketone was crystallized in an orthorhombic crystal form. Orientation and position of a starting model… Expand
Effect of the Z mutation on the physical and inhibitory properties of alpha 1-antitrypsin.
- D. Lomas, D. L. Evans, S. Stone, W. S. Chang, R. Carrell
- Chemistry, Medicine
- 19 January 1993
A major feature of the structure of alpha 1-antitrypsin is a five-stranded A-sheet into which the reactive center loop inserts after cleavage. We describe here the effect of the Z mutation (342Glu to… Expand
cDNA cloning of porcine brain prolyl endopeptidase and identification of the active-site seryl residue.
Prolyl endopeptidase is a cytoplasmic serine protease. The enzyme was purified from porcine kidney, and oligonucleotides based on peptide sequences from this protein were used to isolate a cDNA clone… Expand
Thrombin functions as an inflammatory mediator through activation of its receptor
- G. Cirino, C. Cicala, M. Bucci, L. Sorrentino, J. Maraganore, S. Stone
- The Journal of experimental medicine
- 1 March 1996
A rat model of inflammation was used to investigate the biological effects of thrombin. The thrombin-specific inhibitor Hirulog markedly attentuated the carrageenin-induced edema of the paw of the… Expand
Mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli: pH and deuterium isotope effects with NADPH as the variable substrate.
The variations with pH of the kinetic parameters and primary deuterium isotope effects for the reaction of NADPH with dihydrofolate reductase from Escherichia coli have been determined. The aims of… Expand
Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: evidence for a neuronal-specific isoform.
The trimeric form of protein phosphatase 2A (PP2A1 or polycation-stimulated protein phosphatase H1) was purified to homogeneity from rabbit skeletal muscle. Preparative SDS-polyacrylamide gel… Expand
Proteinase-activated receptor-2: expression by human neutrophils.
Neutrophils were shown to express the proteinase-activated receptor-2 (PAR-2), a seven transmembrane domain receptor, which is activated by cleavage by trypsin. Granulocytes from 14 donors stained… Expand
Kinetics of the inhibition of thrombin by hirudin.
The dissociation constant for hirudin was determined by varying the concentration of hirudin in the presence of a fixed concentration of thrombin and tripeptidyl p-nitroanilide substrate. The… Expand
Thrombin causes neurite retraction in neuronal cells through activation of cell surface receptors
The mechanism by which thrombin induces neurite retraction was studied in NB2a mouse neuroblastoma cells. The rapid effect of thrombin (completed within minutes) appears to involve an interaction… Expand