• Publications
  • Influence
Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor.
A novel microfluidic device that can selectively and specifically isolate exceedingly small numbers of circulating tumor cells (CTCs) through a monoclonal antibody (mAB) mediated process by samplingExpand
Point-of-care biosensor systems for cancer diagnostics/prognostics.
This review seeks to provide an overview of the current thinking on molecular profiling for diagnosis and prognosis of cancers and also, provide insight into the current state-of-the-art in the biosensor field and new strategies that must be considered to bring this important technology into the cancer field. Expand
Surface immobilization methods for aptamer diagnostic applications
In this review we examine various methods for the immobilization of aptamers onto different substrates that can be utilized in a diverse array of analytical formats. In most cases, covalent linkingExpand
Physiochemical properties of various polymer substrates and their effects on microchip electrophoresis performance.
A suite of polymers were evaluated for their suitability as viable substrate materials for microchip electrophoresis applications, which were fabricated via replication technology. The relevantExpand
Surface modification of poly(methyl methacrylate) used in the fabrication of microanalytical devices.
We report here the chemical modification of poly(methyl methacrylate) (PMMA) surfaces by their reaction with the monoanion of alpha,omega-diaminoalkanes (aminolysis reaction) to yieldExpand
Rapid PCR in a continuous flow device.
Continuous flow polymerase chain reaction (CFPCR) devices are compact reactors suitable for microfabrication and the rapid amplification of target DNAs. For a given reactor design, the amplificationExpand
Highly efficient capture and enumeration of low abundance prostate cancer cells using prostate‐specific membrane antigen aptamers immobilized to a polymeric microfluidic device
Prostate tumor cells over‐express a prostate‐specific membrane antigen (PSMA) that can be used as a marker to select these cells from highly heterogeneous clinical samples, even when found in lowExpand
Abstract— The successful implementation of ultrasensitive fluorescence spectroscopy of biological and chemical species depends upon certain photophysical parameters associated with the fluorescentExpand
Simple room temperature bonding of thermoplastics and poly(dimethylsiloxane).
We describe a simple and versatile method for bonding thermoplastics to elastomeric polydimethylsiloxane (PDMS) at room temperature. The bonding of various thermoplastics including polycarbonateExpand
Polymerase chain reaction/ligase detection reaction/hybridization assays using flow-through microfluidic devices for the detection of low-abundant DNA point mutations.
We have microfabricated a flow-through biochip for the analysis of single base mutations in genomic DNA using two different materials: (1) a polycarbonate (PC) chip for performing a primaryExpand