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The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle
TLDR
Genetic evidence indicated that a mutant lacking all three chitin synthases was inviable; this was confirmed by constructing a triple mutant rescued by a plasmid carrying a CHS2 gene under control of a GAL1 promoter.
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Methods in yeast genetics (laboratory course manual): By F. Sherman, G. R. Fink, and J. B. Hicks, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1987. 186 pp. Paper bound, $25.00
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Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae
We developed systems to rapidly express any yeast gene or to specifically degrade any protein, each with minimal untargeted disturbance of cell physiology. We illustrate applications of these new…
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Nikkomycin Z is a specific inhibitor of Saccharomyces cerevisiae chitin synthase isozyme Chs3 in vitro and in vivo
TLDR
Assays of chitin synthase isozymes and growth assays with isozyme mutants were used to demonstrate that nikkomycin Z is a selective inhibitor of chiton synthase 3, likely responsible for the poor activity of this antibiotic against Saccharomyces cerevisiae.
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Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae.
TLDR
The mechanism of in vivo synthesis of chitin has been clarified by cloning the structural gene for the newly found chitIn synthase 2, a relatively minor activity in yeast, establishing that the gene product is essential for both septum formation and cell division.
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Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate
TLDR
The yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.
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Multiple Copies of PBS2, MHP1 or LRE1 Produce Glucanase Resistance and Other Cell Wall Effects in Saccharomyces cerevisiae
TLDR
Five sequences were isolated by selection for multiple copy plasmids that conferred resistance to laminarinase, an enzyme that specifically degrades cell wall β(1‐3) glucan linkages, indicating that the production of these cell wall effects requires the presence of both LRE1 and YCL052C (PBN1).
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Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae
TLDR
It is concluded that damage to the cell wall is caused by excessive chitinase activity at acidic pH, which can normally be repaired through chit in synthesis by Chs1, and the latter emerges as an auxiliary or emergency enzyme.
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Chitinase and chitin synthase 1: counterbalancing activities in cell separation of Saccharomyces cerevisiae.
TLDR
The results indicate that, in addition to chitinase, lysis requires other gene products that may become limiting, such as demethylallosamidin, a much better protector against lysis.
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Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae
TLDR
Transcription can be restored by genetic rearrangements involving the Ty element inserted at HIS4, several deletions, an inversion, a translocation, and a gene conversion are capable of restoring HIS4 transcription.
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