• Publications
  • Influence
Transition State Stabilization by a Catalytic RNA
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of proteinExpand
  • 231
  • 10
  • PDF
Site-specific incorporation of nitroxide spin-labels into internal sites of the TAR RNA; structure-dependent dynamics of RNA by EPR spectroscopy.
RNA can catalyze chemical reactions 1 and interacts in specific ways with other macromolecules, such as proteins. The mechanistic understanding of RNA function relies on structural information;Expand
  • 94
  • 2
  • PDF
Nanometer distance measurements on RNA using PELDOR.
A rapid increase in RNA structural studies has been seen in the past few years, in part due to the interest in the multitude of cellular functions performed by RNA. Electron paramagnetic resonanceExpand
  • 101
  • 2
  • PDF
Conformational flexibility of DNA.
Pulsed Electron-Electron Double Resonance (PELDOR) on double-stranded DNA (ds-DNA) was used to investigate the conformational flexibility of helical DNA. Stretching, twisting, and bending flexibilityExpand
  • 77
  • 2
  • PDF
Noncovalent and site-directed spin labeling of nucleic acids.
Electron paramagnetic resonance (EPR) spectroscopy is widely used to study free radicals or paramagnetic centers associated with biopolymers. With the advent of pulsed EPR methods, which allowExpand
  • 41
  • 2
  • PDF
Structural features and dynamics of a cold‐adapted alkaline phosphatase studied by EPR spectroscopy
EPR spectroscopy, performed after site‐directed spin‐labeling, was used to study structural dynamics in a cold‐adapted alkaline phosphatase (EC 3.1.1.1). Differences in the structural environment ofExpand
  • 18
  • 2
  • PDF
Electron paramagnetic resonance dynamic signatures of TAR RNA-small molecule complexes provide insight into RNA structure and recognition.
Electron paramagnetic resonance (EPR) spectroscopy was utilized to investigate the correlation between RNA structure and RNA internal dynamics in complexes of HIV-1 TAR RNA with small molecules. TARExpand
  • 45
  • 1
  • PDF
Trityl radicals: spin labels for nanometer-distance measurements.
Spin labelling with trityls: to gather information about the structure and dynamics of trityl radicals, spin-labelled polymers were measured with pulsed electron-electron double resonance (PELDOR)Expand
  • 74
  • 1
  • PDF
Synthesis and characterization of RNA containing a rigid and nonperturbing cytidine-derived spin label.
The nitroxide-containing nucleoside Çm is reported as the first rigid spin label for paramagnetic modification of RNA by solid-phase synthesis. The spin label is well accommodated in several RNAExpand
  • 44
  • 1
  • PDF